Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Plaza de Misael Bañuelos s/n, 09001 Burgos, Spain.
Departamento de Biotecnología y Ciencia de los Alimentos, Universidad de Burgos, Plaza de Misael Bañuelos s/n, 09001 Burgos, Spain.
Food Chem. 2021 Apr 16;342:128300. doi: 10.1016/j.foodchem.2020.128300. Epub 2020 Oct 6.
We have developed a new method for the rapid (2 h) and inexpensive (materials cost < 0.02 €/sample) "2-in-1" determination of the total phenolic content (TPC) and the antioxidant activity (AOX) in honey samples. The method is based on hydrophilic colorimetric films with diazonium groups, which react with phenols rendering highly colored azo groups. The TPC of the sample is correlated to its trolox equivalent antioxidant capacity (TEAC). The intensity of the color allows us to determine both TPC and TEAC of the sample by the analysis of a picture taken with a smartphone that is analysed by the use of the color-definition-parameters (RGB). The controlled light conditions and the systematic use of the same camera avoid the periodical calibration of the system improving the efficiency of the method. Thus, it is a simple method carried out by non-specialized personnel and it involves much lower money and time investment compared to traditional methods.
我们开发了一种新方法，可快速（2 小时）且廉价（材料成本<0.02 欧元/样品）地“二合一”测定蜂蜜样品中的总酚含量（TPC）和抗氧化活性（AOX）。该方法基于带有重氮基团的亲水性比色膜，其与酚类反应生成高度着色的偶氮基团。样品的 TPC 与其 Trolox 等效抗氧化能力（TEAC）相关。颜色的强度允许我们通过智能手机拍摄的照片进行分析，该照片通过使用颜色定义参数（RGB）进行分析，从而确定样品的 TPC 和 TEAC。受控的光照条件和系统地使用相同的相机避免了系统的定期校准，从而提高了方法的效率。因此，这是一种由非专业人员进行的简单方法，与传统方法相比，它涉及的资金和时间投入要少得多。
