Department of Neurology, Massachusetts General Hospital, Boston, MA, 02114
Department of Neurology, Massachusetts General Hospital, Boston, MA, 02114.
eNeuro. 2020 Oct 28;7(5). doi: 10.1523/ENEURO.0212-20.2020. Print 2020 Sep/Oct.
electrophysiology experiments require the collection of data from multiple subjects, often for extended periods. Studying multiple subjects for extended periods can be made more efficient through simultaneous recordings, but scaling up recordings to accommodate larger numbers of subjects simultaneously requires coordination and consideration of costs and flexibility. To facilitate this process, we have developed OpBox, an open source set of tools to acquire electroencephalography (EEG) and electromyography (EMG) flexibly from multiple rodent subjects simultaneously. OpBox combines open source hardware and software with off-the-shelf components to create a system that costs less than commercial solutions ($500 per subject), and can be easily deployed for multiple subjects. Coded in MATLAB, OpBox scripts can simultaneously and flexibly collect and display multiple analog and digital data streams, for instance real-time EEG and EMG, event triggers from a behavioral system, and rotary encoder data. OpBox also calculates and displays real-time spectral representations and event-related potentials (ERPs). To verify the performance of our system, we compare our amplifiers with two other commercial amplifiers, a Grass P55 AC preamplifier and an Intan RHD2000-series amplifier. The OpBox amplifier performs comparably to commercial amplifiers for signal-to-noise ratios (SNRs), noise floors, and common mode rejection. We also demonstrate that our acquisition system can reliably record multichannel data from multiple subjects, and has been successfully tested with 12 subjects running simultaneously on a single standard desktop computer. Together, OpBox increases the flexibility and lowers the cost for simultaneous acquisition of electrophysiology data from multiple subjects.
电生理学实验需要从多个被试者收集数据，通常需要较长时间。通过同时记录可以提高对多个被试者进行长时间研究的效率，但要扩大记录规模以同时容纳更多的被试者，则需要协调并考虑成本和灵活性。为了促进这一过程，我们开发了 OpBox，这是一套开源工具，用于灵活地从多个啮齿动物被试者同时获取脑电图 (EEG) 和肌电图 (EMG)。OpBox 将开源硬件和软件与现成组件相结合，创建了一个成本低于商业解决方案的系统（每个被试者 500 美元），并且可以轻松地部署用于多个被试者。OpBox 的代码是用 MATLAB 编写的，可以同时灵活地收集和显示多个模拟和数字数据流，例如实时 EEG 和 EMG、来自行为系统的事件触发以及旋转编码器数据。OpBox 还可以计算和显示实时频谱表示和事件相关电位 (ERPs)。为了验证我们系统的性能，我们将我们的放大器与另外两个商业放大器，即 Grass P55 AC 前置放大器和 Intan RHD2000 系列放大器进行了比较。OpBox 放大器在信噪比 (SNR)、噪声底和共模抑制方面与商业放大器性能相当。我们还证明了我们的采集系统可以可靠地从多个被试者记录多通道数据，并且已经在单个标准台式计算机上同时运行 12 个被试者的情况下成功进行了测试。总之，OpBox 提高了同时从多个被试者获取电生理数据的灵活性并降低了成本。
