从临床广泛耐药的ST648中分离出的携带新型IncHI2质粒pE648CTX-M-65 。
A Novel -Harboring IncHI2 Plasmid pE648CTX-M-65 Isolated from a Clinical Extensively-Drug-Resistant ST648.
作者信息
Lü Yang, Kang Haiquan, Fan Jianming
机构信息
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Department of Laboratory Medicine, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, People's Republic of China.
出版信息
Infect Drug Resist. 2020 Sep 30;13:3383-3391. doi: 10.2147/IDR.S269766. eCollection 2020.
BACKGROUND
An ESBL, carbapenemase- and MCR-1-producing ST648 strain was isolated from the urine sample of a patient in a Chinese tertiary hospital in 2016.
METHODS
The strain was fully sequenced by GridION X5 platform of Oxford Nanopore Technology.
RESULTS
The sequence analysis showed that the extended-spectrum β-lactamases CTX-M-65 and OXA-1, the carbapenemase NDM-5, the MCR-1 were encoded, respectively, by three different resistance plasmids. The pE648CTX-M-65-carrying was a novel conjugative plasmid belonging to IncHI2 type; except for the , it also carried resistance genes , and . Besides, an IncX4 plasmid pE648MCR-1-carrying and an IncX3 plasmid pE648NDM-5-carrying were also identified.
CONCLUSION
The three transferable resistance plasmids coexisting in the ST648 isolate indicated the high risk to disseminate the extensively-drug-resistance among Enterobacteriaceae.
背景
2016年，在中国一家三级医院的一名患者尿液样本中分离出一株产超广谱β-内酰胺酶（ESBL）、碳青霉烯酶和MCR-1的ST648菌株。
方法
采用牛津纳米孔技术的GridION X5平台对该菌株进行全基因组测序。
结果
序列分析表明，超广谱β-内酰胺酶CTX-M-65和OXA-1、碳青霉烯酶NDM-5、MCR-1分别由三种不同的耐药质粒编码。携带pE648CTX-M-65的质粒是一种属于IncHI2型的新型接合质粒；除了 ，它还携带耐药基因 、 和 。此外，还鉴定出一个携带IncX4质粒pE648MCR-1的质粒和一个携带IncX3质粒pE648NDM-5的质粒。
结论
ST648分离株中三种可转移耐药质粒共存，表明肠杆菌科细菌广泛耐药传播的高风险。
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