Muthoni Guchu Beatrice, Machocho Alex King'ori, Mwihia Stephen Kiruthi, Ngugi Mathew Piero
Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, P. O. Box 43844-00100, Nairobi, Kenya.
Department of Chemistry, School of Pure and Applied Sciences, Kenyatta University, P. O. Box 43844-00100, Nairobi, Kenya.
Evid Based Complement Alternat Med. 2020 Sep 27;2020:3586268. doi: 10.1155/2020/3586268. eCollection 2020.
Oxidative stress is the result of the disparity between pro-oxidants and antioxidants in an organism, and it is important in the pathogenesis of several degenerative disorders, such as arthritis, Alzheimer's, cancer, and cardiovascular diseases. Free radicals can damage biomolecules, such as nucleic acids, lipids, proteins, polyunsaturated fatty acids, and carbohydrates, and the DNA leading to mutations. The use of antioxidants is effective in delaying the oxidation of biomolecules. Antioxidants are complexes found in the food that can retard or deter oxidation by preventing the initiation and propagation of oxidizing chain reactions. Medicinal plants have been used for centuries by man to manage diseases and have a host of antioxidant complexes. Traditionally, and have folkloric remedies against associated oxidative stress-mediated complications. However, the upsurge in its use has not been accompanied by scientific validations to support these claims. In this study, in vitro antioxidant activity of and collected from Embu County (Kenya) were determined by radical scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical in addition to ferric reducing antioxidant power analyzed against that of L-ascorbic acid as the standard. The obtained results revealed remarkable antioxidant activities of the studied plant extracts as evidenced by the low IC50 and EC50 values. These antioxidant activities could be due to the presence of antioxidants phytochemicals such as flavonoids, phenols, terpenoids, and saponins among others. Therefore, the therapeutic potential of this plant could be due to their antioxidant properties. This study recommends bioassay of the extracts against oxidative stress-related disorders for development of phytomedicine with antioxidant properties.
氧化应激是生物体中促氧化剂和抗氧化剂之间失衡的结果，在几种退行性疾病的发病机制中起重要作用，如关节炎、阿尔茨海默病、癌症和心血管疾病。自由基会损害生物分子，如核酸、脂质、蛋白质、多不饱和脂肪酸和碳水化合物，以及导致突变的DNA。使用抗氧化剂可有效延缓生物分子的氧化。抗氧化剂是食物中发现的复合物，可通过阻止氧化链反应的引发和传播来延缓或抑制氧化。几个世纪以来，人类一直使用药用植物来治疗疾病，药用植物含有大量抗氧化复合物。传统上，[植物名称1]和[植物名称2]有针对相关氧化应激介导并发症的民间疗法。然而，其使用的增加并未伴随着科学验证来支持这些说法。在本研究中，通过1,1-二苯基-2-苦基肼（DPPH）和羟基自由基的自由基清除活性，以及以L-抗坏血酸为标准分析的铁还原抗氧化能力，测定了从肯尼亚恩布县采集的[植物名称1]和[植物名称2]的体外抗氧化活性。获得的结果显示，所研究的植物提取物具有显著的抗氧化活性，低IC50和EC50值证明了这一点。这些抗氧化活性可能归因于抗氧化植物化学物质的存在，如黄酮类、酚类、萜类和皂苷等。因此，这种植物的治疗潜力可能归因于其抗氧化特性。本研究建议对提取物针对氧化应激相关疾病进行生物测定，以开发具有抗氧化特性的植物药。
