Muruthi Carolyn Wanjira, Ngugi Mathew Piero, Runo Steven Maina, Mwitari Peter Githaiga
Department of Biochemistry, Microbiology and Biotechnology-Kenyatta University, Kenya.
Centre for Traditional Medicine and Drug Research-Kenya Medical Research Institute (KEMRI), Kenya.
Heliyon. 2023 Jan 19;9(1):e12965. doi: 10.1016/j.heliyon.2023.e12965. eCollection 2023 Jan.
Herbal medications are gaining popularity due to their long history of use in traditional medicine. They serve as a reservoir for a diverse array of phytocompounds linked to amelioration of oxidative stress. Oxidative stress is a disturbance in the balance between generation and elimination of reactive species in human body. Moreover, reactive species are implicated in the onset and progression of chronic disorders. The current therapeutic approaches despite showing efficacy are characterized by several limitations such as adverse effects and prohibitive costs. This drives the need to explore alternatives that can inhibit, ameliorate or reverse conditions caused by oxidative stress. Several studies have evaluated antioxidant effects of diverse plant extracts. and are used as traditional therapy among the African communities to manage oxidative stress-related ailments. However, there is limited research on the antioxidant effects of these medicinal plants. The current study, therefore, sought to evaluate the antioxidant and phytochemical profile, of and extracts. Samples were collected from Embu County, Kenya. antioxidant properties of the extracts were evaluated through ferric reduction, Iron chelating, hydroxyl radical, and DPPH radical scavenging activities. Activities of catalase, superoxide dismutase and glutathione reductases of the extracts were further determined. Phytochemical profiles were determined using Liquid Chromatography Mass Spectrophotometer (LC-MS) and Gas Chromatography Mass Spectrophotometer (GC-MS) analyses. The extracts displayed concentration dependent antioxidant activities. Phytochemical analyses revealed presence compounds which are associated with antioxidant activities including flavonoids, phenolics, tocopherols and terpenoids. The findings provide a scientific validation for the folklore use of and in management of oxidative stress. Nevertheless, there is a need for further purification and characterization of phytochemicals associated with antioxidant activities.
草药因其在传统医学中的悠久使用历史而越来越受欢迎。它们是与减轻氧化应激相关的多种植物化合物的储存库。氧化应激是人体中活性物质产生与消除之间平衡的紊乱。此外，活性物质与慢性疾病的发生和发展有关。目前的治疗方法尽管显示出疗效，但具有一些局限性，如副作用和高昂的成本。这促使人们需要探索能够抑制、减轻或逆转由氧化应激引起的病症的替代方法。多项研究评估了多种植物提取物的抗氧化作用。[植物名称1]和[植物名称2]在非洲社区被用作传统疗法来管理与氧化应激相关的疾病。然而，关于这些药用植物抗氧化作用的研究有限。因此，本研究旨在评估[植物名称1]和[植物名称2]提取物的抗氧化和植物化学特征。样本采集自肯尼亚的恩布县。通过铁还原、铁螯合、羟基自由基和DPPH自由基清除活性来评估提取物的抗氧化性能。进一步测定提取物中过氧化氢酶、超氧化物歧化酶和谷胱甘肽还原酶的活性。使用液相色谱 - 质谱仪（LC - MS）和气相色谱 - 质谱仪（GC - MS）分析来确定植物化学特征。提取物表现出浓度依赖性的抗氧化活性。植物化学分析揭示了存在与抗氧化活性相关的化合物，包括黄酮类、酚类、生育酚和萜类化合物。这些发现为[植物名称1]和[植物名称2]在民间用于管理氧化应激提供了科学验证。然而，仍需要进一步纯化和表征与抗氧化活性相关的植物化学物质。
