Zhang Kai-Hui, Wang Mei-Qi, Wei Lu-Ling, Feng Cheng-Jing, Zhang Yu-Si, Teng Jian-Bei
College of Pharmaceutical Science, Guangxi University of Chinese Medicine, Nanning 530200, China.
Evid Based Complement Alternat Med. 2020 Sep 30;2020:4367347. doi: 10.1155/2020/4367347. eCollection 2020.
Gout is a chronic disease that causes inflammatory arthritis, which is closely related to urate accumulation induced by a disorder of uric acid metabolism and the consequent deposition of monosodium urate crystals. Rolfe is an herbal medicine that has been used in some traditional Chinese medicine formulae in the treatment of gout. This study aimed to explore and verify the antigout activity of extract (DLE) on alleviating the hyperuricaemia of mice and the acute gouty arthritis of rats.
An animal model of hyperuricaemia was established using potassium oxonate (PO). We analysed the expression of uric acid transporter mRNA in the kidney in the hyperuricaemic mice after treatment with DLE. Simultaneously, a monosodium urate crystal-induced acute gouty arthritis rat model was used to evaluate the effects of DLE, according to the level of ankle swelling, as well as the protein levels of inflammatory receptors and cytokines, as assayed by WB and ELISA.
DLE alleviated hyperuricaemia in mice and inhibited acute gouty arthritis in rats ( < 0.05). Meanwhile, DLE regulated the levels of uric acid transporters mRNA transcripts, including mouse organic anion transporter 1 (mOAT1), organic anion transporter 3 (mOAT3), urate transporter 1 (mURAT1), and glucose transporter 9 (mGLUT9) in the kidney ( < 0.05), suggesting that DLE promoted uric acid metabolism. Furthermore, DLE significantly suppressed the protein levels of TLRs, MyD88, and NF-B in the ankle joint's synovium ( < 0.05), and the serum levels of IL-1, IL-6, and TNF- were also reduced, which demonstrated the anti-inflammatory effects of DLE.
DLE alleviates hyperuricaemia by regulating the transcription level of uric acid transporters in the kidney. It also inhibits acute gouty arthritis by inhibiting the pathway of TLRs/MyD88/NF-B in the ankle joint's synovium. The findings of the present study imply that DLE alleviates gout by promoting uric acid metabolism and inhibiting inflammation related to the TLRs/MyD88/NF-B pathway.
痛风是一种导致炎性关节炎的慢性疾病，与尿酸代谢紊乱引起的尿酸盐积聚以及随后的尿酸钠晶体沉积密切相关。萝芙木是一种已被用于一些中药配方中治疗痛风的草药。本研究旨在探索并验证萝芙木提取物（DLE）对减轻小鼠高尿酸血症和大鼠急性痛风性关节炎的抗痛风活性。
使用氧嗪酸钾（PO）建立高尿酸血症动物模型。我们分析了DLE处理后高尿酸血症小鼠肾脏中尿酸转运蛋白mRNA的表达。同时，使用尿酸钠晶体诱导的急性痛风性关节炎大鼠模型，根据踝关节肿胀程度以及通过蛋白质免疫印迹法（WB）和酶联免疫吸附测定法（ELISA）检测的炎性受体和细胞因子的蛋白质水平来评估DLE的作用。
DLE减轻了小鼠的高尿酸血症并抑制了大鼠的急性痛风性关节炎（P<0.05）。同时，DLE调节了肾脏中尿酸转运蛋白mRNA转录本的水平，包括小鼠有机阴离子转运体1（mOAT1）、有机阴离子转运体3（mOAT3）、尿酸转运体1（mURAT1）和葡萄糖转运体9（mGLUT9）（P<0.05），表明DLE促进了尿酸代谢。此外，DLE显著抑制了踝关节滑膜中Toll样受体（TLRs）、髓样分化因子88（MyD88）和核因子κB（NF-κB）的蛋白质水平（P<0.05），并且血清中白细胞介素-1（IL-1）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平也降低了，这证明了DLE的抗炎作用。
DLE通过调节肾脏中尿酸转运蛋白的转录水平来减轻高尿酸血症。它还通过抑制踝关节滑膜中TLRs/MyD88/NF-κB通路来抑制急性痛风性关节炎。本研究结果表明，DLE通过促进尿酸代谢和抑制与TLRs/MyD88/NF-κB通路相关的炎症来减轻痛风。
