Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China.
Genes (Basel). 2024 Jun 25;15(7):834. doi: 10.3390/genes15070834.
is a precious traditional Chinese medicine with high medicinal and ornamental value. However, the characterization of its mitochondrial genome is still pending. Here, we assembled the complete mitochondrial genome of and discovered that its genome possessed a complex multi-chromosome structure. The mitogenome of consisted of 17 circular subgenomes, ranging in size from 16,323 bp to 56,781 bp. The total length of the mitogenome was 513,356 bp, with a GC content of 43.41%. The mitogenome contained 70 genes, comprising 36 protein-coding genes (PCGs), 31 tRNA genes, and 3 rRNA genes. Furthermore, we detected 403 repeat sequences as well as identified 482 RNA-editing sites and 8154 codons across all PCGs. Following the sequence similarity analysis, 27 fragments exhibiting homology to both the mitogenome and chloroplast genome were discovered, accounting for 9.86% mitogenome of . Synteny analysis revealed numerous sequence rearrangements in and the mitogenomes of related species. Phylogenetic analysis strongly supported that and formed a single clade with 100% bootstrap support. The outcomes will significantly augment the orchid mitochondrial genome database, offering profound insights into s intricate mitochondrial genome architecture.
是一种具有高药用和观赏价值的珍贵中药。然而，其线粒体基因组的特征仍有待研究。在这里，我们组装了的完整线粒体基因组，并发现其基因组具有复杂的多染色体结构。的线粒体基因组由 17 个圆形亚基因组组成，大小从 16323bp 到 56781bp 不等。线粒体基因组的总长度为 513356bp，GC 含量为 43.41%。线粒体基因组包含 70 个基因，包括 36 个蛋白质编码基因（PCGs）、31 个 tRNA 基因和 3 个 rRNA 基因。此外，我们检测到 403 个重复序列，并在所有 PCGs 中鉴定出 482 个 RNA 编辑位点和 8154 个密码子。通过序列相似性分析，发现了 27 个与线粒体基因组和叶绿体基因组都具有同源性的片段，占的线粒体基因组的 9.86%。同线性分析显示在和相关物种的线粒体基因组中存在许多序列重排。系统发育分析强烈支持和形成一个单系群，支持率为 100%。这些结果将极大地丰富兰花线粒体基因组数据库，深入了解的复杂线粒体基因组结构。
