Welsh Joshua A, van der Pol Edwin, Bettin Britta A, Carter David R F, Hendrix An, Lenassi Metka, Langlois Marc-André, Llorente Alicia, van de Nes Arthur S, Nieuwland Rienk, Tang Vera, Wang Lili, Witwer Kenneth W, Jones Jennifer C
Translational Nanobiology Section, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, USA.
Vesicle Observation Center, Amsterdam UMC, Location AMC, University of Amsterdam, Amsterdam, The Netherlands.
J Extracell Vesicles. 2020 Sep 24;9(1):1816641. doi: 10.1080/20013078.2020.1816641.
Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of reference materials are required for the standardization of these techniques. This work, initiated from the ISEV 2017 Biomarker Workshop in Birmingham, UK, and with further discussion during the ISEV 2019 Standardization Workshop in Ghent, Belgium, sets out to elucidate which reference materials are required and which are currently available to standardize commonly used analysis platforms for characterizing EV refractive index, epitope abundance, size and concentration. Due to their predominant use among EV researchers, a particular focus is placed on the optical methods nanoparticle tracking analysis and flow cytometry.
准确表征细胞外囊泡（EVs）对于探索其诊断和治疗应用至关重要。随着EV研究领域的发展，用于表征它们的技术也在不断发展。这些技术的标准化需要参考材料的开发。这项工作始于2017年在英国伯明翰举行的ISEV生物标志物研讨会，并在2019年于比利时根特举行的ISEV标准化研讨会上进行了进一步讨论，旨在阐明标准化用于表征EV折射率、表位丰度、大小和浓度的常用分析平台需要哪些参考材料以及目前有哪些可用的参考材料。由于它们在EV研究人员中使用广泛，因此特别关注光学方法纳米颗粒跟踪分析和流式细胞术。
