Bell Sara E, Nash Andrea K, Zanghi Brian M, Otto Cynthia M, Perry Erin B
Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, United States.
Penn Vet Working Dog Center, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, United States.
Front Vet Sci. 2020 Sep 11;7:616. doi: 10.3389/fvets.2020.00616. eCollection 2020.
The administration of an oral probiotic has been demonstrated to impact oral microbial diversity in humans but has not been examined in canines. The objective of this study was to test the hypothesis that oral probiotic administration would impact the oral microbiota of canines compared to control. Working canines in training ( = 13) were assigned to Test or Control groups and acclimated to one of three commercially available study diets utilizing common protein sources (Purina Pro Plan Savor lamb, Purina Pro Plan Sport chicken, Purina Pro Plan Focus salmon) for a minimum of 30 days prior to initiation of the study. Following acclimation, dogs in the Test group began a daily regimen of oral probiotic (Fortiflora® Purina, St. Louis, MO) top-dressed on their midday feeding. Control dogs received their midday feeding with no probiotic. All dogs were sampled once weekly via oral pediatric swabs across the 7-week study. Next generation sequencing (Illumina, MiSeq) was utilized to develop microbial profiles specific to treatment, diet, and time. Bacterial composition was dominated by eight phyla (Proteobacteria 43.8%, Bacteroidetes 22.5%, Firmicutes 18.9%, Actinobacteria 6.1%, Fusobacteria 3.6%, Gracilibacteria 2.1%, SR1 Absconditabacteria 1.5%, and Saccharibacteria 1.3%) representing more than 99% of the relative abundance of the microbial composition. Probiotic administration failed to impact relative abundance at any taxonomic level ( > 0.05). Similarly, no effect on the oral microbiota was measured for diet ( > 0.05). Comparison using a Jaccard Index demonstrate a consistent microbial profile over the 7-week study with no impact evidenced by study week ( = 0.19). The data also revealed a profile of ubiquitous taxa that were present across all dogs and all samples regardless of breed, sex, diet, treatment or other factors. These genera include . These data demonstrate the stability of canine oral microbiota over time.
口服益生菌已被证明会影响人类口腔微生物多样性，但尚未在犬类中进行研究。本研究的目的是检验以下假设：与对照组相比，口服益生菌会影响犬类的口腔微生物群。将接受训练的工作犬（n = 13）分为试验组或对照组，并在研究开始前至少30天适应三种使用常见蛋白质来源的市售研究日粮之一（普瑞纳专业配方美味羊肉粮、普瑞纳专业配方运动鸡肉粮、普瑞纳专业配方聚焦三文鱼粮）。适应期过后，试验组的犬只开始在每日中午喂食时添加口服益生菌（普瑞纳Fortiflora®，密苏里州圣路易斯）。对照组犬只中午喂食时不添加益生菌。在为期7周的研究中，所有犬只每周通过口腔儿科拭子采样一次。利用下一代测序（Illumina，MiSeq）来建立特定于治疗、饮食和时间的微生物图谱。细菌组成以八个门为主（变形菌门43.8%、拟杆菌门22.5%、厚壁菌门18.9%、放线菌门6.1%、梭杆菌门3.6%、薄壁菌门2.1%、SR1隐秘杆菌门1.5%、糖杆菌门1.3%），占微生物组成相对丰度的99%以上。益生菌给药在任何分类水平上均未影响相对丰度（P>0.05）。同样，饮食对口腔微生物群也没有影响（P>0.05）。使用杰卡德指数进行比较表明，在为期7周的研究中微生物图谱一致，研究周数未显示出影响（P = 0.19）。数据还揭示了一个普遍存在的分类群图谱，无论品种、性别、饮食、治疗或其他因素如何，所有犬只和所有样本中均存在这些分类群。这些属包括……这些数据证明了犬类口腔微生物群随时间的稳定性。
