Kallas Pawel, Kang Hua, Valen Håkon, Haugen Håvard Jostein, Andersson Martin, Hulander Mats
Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway.
Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Biomater Investig Dent. 2020 Sep 15;7(1):134-145. doi: 10.1080/26415275.2020.1816175.
This study investigated the effect of surface nano-patterning on adhesion of an oral early commensal colonizer, and the opportunistic pathogen and human fibroblasts (HDFa) in a laminar flow cell.
Nanostructured surfaces were made by functionalizing glass substrates with 40 nm SiO nanoparticles. Gradients in nanoparticle surface coverage were fabricated to study the effect of nanoparticle spacing within a single experiment. Bacterial adhesion was investigated after 5 min of contact time by subjecting surfaces to a flow in a laminar flow cell. In addition, to examine the particles effect on human cells, the establishment of focal adhesion and spreading of primary human dermal fibroblasts (HDFa) were investigated after 4 and 24 h.
Adhesion of both and decreased on surfaces functionalized with nanoparticles and coincided with higher nanoparticle surface coverage on the surface. Both strains were tested on three separate surfaces. The regression analysis showed that was influenced more by surface modification than . The establishment of focal adhesions in HDFa cells was delayed on the nanostructured part of the surfaces after both 4 and 24 h of culturing.
In the current manuscript, we have used a flow cell to investigate the effect of nanotopographies on and adhesion. The present findings are of relevance for design of future implant and prostheses surfaces in order to reduce adhesion of bacteria.
本研究在层流流动小室中，探究表面纳米图案化对一种口腔早期共生定植菌、一种机会致病菌以及人成纤维细胞（HDFa）黏附的影响。
通过用40纳米的二氧化硅纳米颗粒对玻璃基板进行功能化处理来制备纳米结构表面。制造纳米颗粒表面覆盖率的梯度，以便在单个实验中研究纳米颗粒间距的影响。在层流流动小室中使表面流动5分钟后，研究细菌黏附情况。此外，为了检测颗粒对人细胞的影响，在培养4小时和24小时后，研究原代人皮肤成纤维细胞（HDFa）的粘着斑形成和铺展情况。
在用纳米颗粒功能化的表面上，两种菌的黏附均减少，且与表面上更高的纳米颗粒表面覆盖率一致。两种菌株均在三个不同表面上进行了测试。回归分析表明，表面修饰对[某种菌]的影响比对[另一种菌]的影响更大。在培养4小时和24小时后，HDFa细胞在表面的纳米结构部分粘着斑的形成均延迟。
在本论文中，我们使用流动小室来研究纳米拓扑结构对[两种菌]黏附的影响。目前的研究结果对于未来植入物和假体表面的设计具有重要意义，以便减少细菌黏附。
