Department of Orthopedic Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, Germany.
Institute of Materials and Joining Technology, Otto-von-Guericke University Magdeburg, Magdeburg, Germany.
J Appl Microbiol. 2021 Sep;131(3):1498-1514. doi: 10.1111/jam.15033. Epub 2021 Mar 11.
Joint infections cause premature implant failure. The avoidance of bacterial colonization of implant materials by modification of the material surface is therefore the focus of current research. In this in vitro study the complex interaction of periodic structures on PET and titanium surfaces on the adhesion of Staphylococcus aureus is analysed.
Using direct laser interference patterning as well as roll-to-roll hot embossing methods, structured periodic textures of different spatial distance were produced on surfaces and S. aureus were cultured for 24 h on these. The amount of adhering bacteria was quantified using fluorescence microscopy and the local adhesion behaviour was investigated using scanning electron microscopy. For PET structures, minimal bacterial adhesion was identified for an aspect ratio of about 0·02. On titanium structures, S. aureus adhesion was significantly decreased for profile heights of < 200 nm. Our results show a significantly decreased bacterial adhesion for structures with an aspect ratio range of 0·02 to 0·05.
We show that structuring on surfaces can decrease the amount of S. aureus on titanium and PET as common implant materials.
The study highlights the immense potential of applying specific structures to implant materials to prevent implant colonization with pathogen bacteria.
关节感染会导致植入物提前失效。因此，目前的研究重点是通过改变材料表面来避免细菌在植入物材料上的定植。本体外研究分析了 PET 和钛表面周期性结构的复杂相互作用对金黄色葡萄球菌黏附的影响。
使用直接激光干涉图案化以及卷对卷热压印方法，在表面上制作了不同空间距离的结构化周期性纹理，并在这些表面上培养金黄色葡萄球菌 24 小时。使用荧光显微镜定量测定黏附的细菌数量，并使用扫描电子显微镜研究局部黏附行为。对于 PET 结构，当纵横比约为 0.02 时，细菌黏附最少。对于钛结构，当轮廓高度<200nm 时，金黄色葡萄球菌的黏附显著减少。我们的结果表明，具有 0.02 至 0.05 纵横比范围的结构可显著减少金黄色葡萄球菌的黏附。
我们表明，在表面上进行结构化处理可以减少钛和 PET 等常见植入物材料上金黄色葡萄球菌的数量。
该研究强调了将特定结构应用于植入物材料以防止病原体细菌定植的巨大潜力。
