FARAH Center, Comparative Veterinary Medicine Section, Clinical Department of Companion Animals and Equids, Faculty of Veterinary Medicine, University of Liege, Liège, Belgium.
FARAH Center, Veterinary Public Health Section, Department of Food Sciences - Microbiology, Faculty of Veterinary Medicine, University of Liege, Liège, Belgium.
J Vet Intern Med. 2020 Nov;34(6):2727-2737. doi: 10.1111/jvim.15937. Epub 2020 Oct 16.
Omeprazole administration is associated with changes in gastric and fecal microbiota and increased incidence of Clostridioides difficile enterocolitis in humans and dogs.
HYPOTHESIS/OBJECTIVES: Study purpose was to assess the effect of omeprazole on gastric glandular and fecal microbiota in healthy adult horses.
Eight healthy horses stabled on straw and fed 100% haylage.
Prospective controlled study. Transendoscopic gastric glandular biopsies, gastric fluid, and fecal samples were obtained from each horse twice at a 7-day interval before the administration of omeprazole. Samples were taken on the same horses before and after a 7-day administration of omeprazole (4 mg/kg PO q24h). pH was assessed on fresh gastric fluid and other samples were kept at -20°C until analysis. Bacterial taxonomy profiling was obtained by V1V3 16S amplicon sequencing from feces and gastric glandular biopsies. Analysis of alpha, beta diversity, and comparison between time points were performed with MOTHUR and results were considered significant when P < .05.
Gastric pH increased significantly after 7 days of omeprazole administration (P = .006). Omeprazole did not induce significant major changes in composition of fecal or gastric glandular microbiota, however, after administration, certain microbial genera became more predominant in the gastric glandular mucosa (lower Simpson's evenness, P = .05). Only the genus Clostridium sensu strictu_1 had a significant shift in the glandular gastric mucosa after omeprazole administration (P = .002). No population shifts were observed in feces.
Oral administration of omeprazole could have fewer effects in gastrointestinal microbiota in the horse compared to other species.
奥美拉唑的应用与人类和犬类的胃和粪便微生物群的变化以及艰难梭菌相关性肠炎的发病率增加有关。
假设/目的：本研究旨在评估奥美拉唑对健康成年马胃腺和粪便微生物群的影响。
8 匹在稻草上饲养并以干草为主食的健康成年马。
前瞻性对照研究。在给予奥美拉唑前，每匹马两次经内镜获取胃腺活检、胃液和粪便样本，两次间隔为 7 天。在给予奥美拉唑（4 mg/kg PO q24h）7 天后，在相同的马身上采集相同的样本。新鲜胃液进行 pH 值评估，其余样本在 -20°C 下保存直至分析。通过粪便和胃腺活检的 V1V3 16S 扩增子测序获得细菌分类群谱。使用 MOTHUR 进行 alpha、beta 多样性分析和时间点之间的比较，当 P<.05 时认为结果有统计学意义。
奥美拉唑给药 7 天后，胃 pH 值显著升高（P =.006）。奥美拉唑并未引起粪便或胃腺微生物群组成的显著重大变化，但给药后，某些微生物属在胃腺黏膜中更为优势（较低的辛普森均匀度，P =.05）。只有严格意义上的梭菌属在奥美拉唑给药后胃腺黏膜中发生了显著的转移（P =.002）。粪便中未观察到种群转移。
与其他物种相比，奥美拉唑在马的胃肠道微生物群中可能具有较少的作用。
