Aoki E, Semba R, Kato K, Kashiwamata S
Neuroscience. 1987 Jun;21(3):755-65. doi: 10.1016/0306-4522(87)90035-2.
The distribution of L-aspartate known as a putative excitatory neurotransmitter in the central nervous system was investigated immunocytochemically in the rat brain. Anti-aspartate antiserum was raised in rabbits using L-aspartate covalently conjugated to rabbit serum albumin with glutaraldehyde as the immunogen and was found to be cross-reactive with an L-glutamate conjugate. Monospecific anti-L-aspartate antibody was successfully purified using affinity gels coupled with several amino acids including L-aspartate and L-glutamate and with the L-glutamate conjugate. Putative aspartergic neurons were generally immunoreactive to the purified antibody, but epithelia of the choroid plexus were also stained. These results show that the antibody is a useful tool for the immunocytochemical demonstration of possible aspartergic neurons in the central nervous system, although the immunochemical expression of L-aspartate not used as a neurotransmitter must be taken into consideration.
利用免疫细胞化学方法，在大鼠脑中研究了作为假定兴奋性神经递质的L-天冬氨酸在中枢神经系统中的分布。以戊二醛共价偶联L-天冬氨酸与兔血清白蛋白作为免疫原，在兔体内制备抗天冬氨酸抗血清，发现其与L-谷氨酸偶联物有交叉反应。使用与包括L-天冬氨酸、L-谷氨酸及L-谷氨酸偶联物在内的多种氨基酸偶联的亲和凝胶，成功纯化出单特异性抗L-天冬氨酸抗体。假定的天冬氨酸能神经元通常对纯化抗体呈免疫反应，但脉络丛上皮也被染色。这些结果表明，该抗体是用于中枢神经系统中可能的天冬氨酸能神经元免疫细胞化学示踪的有用工具，不过必须考虑未用作神经递质的L-天冬氨酸的免疫化学表达。
