Campistron G, Buijs R M, Geffard M
Brain Res. 1986 Feb 12;365(1):179-84. doi: 10.1016/0006-8993(86)90737-7.
An immunological approach to visualize aspartate in the rat brain was attempted by raising antibodies against this acidic amino acid. Using an adapted ELISA method, their specificity was tested by competition experiments between aspartate conjugated via glutaraldehyde to various protein-carriers and either non-conjugated aspartate or conjugated amino acids, preincubated with anti-aspartate antibodies. Their titer and specificity were found high enough to allow their use in immunocytochemistry which demonstrated the presence of a large number of aspartate-containing cell-bodies in many areas of the brain.
通过制备针对这种酸性氨基酸的抗体，尝试采用免疫学方法可视化大鼠脑中的天冬氨酸。使用一种改良的酶联免疫吸附测定（ELISA）方法，通过戊二醛偶联到各种蛋白质载体上的天冬氨酸与未偶联的天冬氨酸或与抗天冬氨酸抗体预温育的偶联氨基酸之间的竞争实验，测试了它们的特异性。发现它们的效价和特异性足够高，足以用于免疫细胞化学，该方法证明了大脑许多区域存在大量含天冬氨酸的细胞体。
