Oncology, Onevet-Hospital Veterinário Berna, Lisbon, Portugal.
Faculdade de Medicina Veterinária, Universidade Lusófona de Humanidades e Tecnologias, Lisbon, Portugal.
Vet Comp Oncol. 2021 Mar;19(1):183-190. doi: 10.1111/vco.12658. Epub 2020 Nov 3.
Lymphoma is the most common haematological malignancy in dogs and its aetiology is largely unknown. The presence of canine vector-borne agents (CVBD) in lymphoma tissues has been described and its causative effects questioned. We intended to evaluate the presence and extent of Leishmania infantum, Ehrlichia canis, Anaplasma phagocytophilum and Bartonella henselae infection in dogs with lymphoma. Sixty-one dogs, living in the Lisbon metropolitan area, with a diagnosis of lymphoma were enrolled. Immunofluorescence assays were used to detect serum IgG's. The presence of DNA from CVBD agents in tumour tissue was assessed by PCR. All dogs tested negative for B. henselae, A. phagocytophilum and E. canis by both serology and PCR. Regarding L. infantum, 8.2% (n = 5) of the dogs had a positive serologic result. L. infantum DNA was detected in two samples of diffuse large B-cell lymphoma (DLBCL). These results show an increased, but not significant, seropositivity (8.2% vs 7.9%) and molecular detection (3.3% vs 1.2%) for L. infantum in dogs with lymphoma, when compared to the reported canine population in the same geographical area. We could not identify an association between lymphoma and E. canis, A. phagocytophilum, B. henselae or Leishmania infantum infection in the studied population. Nevertheless, further studies, following dogs trough their CVBD disease evolution, are worthwhile and may help clarify a possible role of CVBD agents in lymphomagenesis.
淋巴瘤是犬类最常见的血液恶性肿瘤，其病因在很大程度上尚不清楚。已经描述了淋巴瘤组织中存在犬媒介传播病原体（CVBD），并质疑其致病作用。我们旨在评估患有淋巴瘤的犬中利什曼原虫、犬埃立克体、嗜吞噬细胞无形体和巴尔通体感染的存在和程度。 共有 61 只生活在里斯本大都市区、被诊断患有淋巴瘤的犬被纳入研究。使用免疫荧光测定法检测血清 IgG。通过 PCR 评估肿瘤组织中 CVBD 病原体 DNA 的存在。所有犬均通过血清学和 PCR 检测均为巴尔通体、嗜吞噬细胞无形体和犬埃立克体阴性。关于利什曼原虫，8.2%（n=5）的犬血清学检测结果为阳性。在 2 例弥漫性大 B 细胞淋巴瘤（DLBCL）样本中检测到利什曼原虫 DNA。这些结果显示，与同一地理区域报告的犬群相比，患有淋巴瘤的犬中利什曼原虫的血清阳性率（8.2%对 7.9%）和分子检测率（3.3%对 1.2%）略有增加，但无统计学意义。我们无法确定在研究人群中，淋巴瘤与犬埃立克体、嗜吞噬细胞无形体、巴尔通体或利什曼原虫感染之间存在关联。然而，对 CVBD 疾病进展中的犬进行进一步研究是值得的，这可能有助于阐明 CVBD 病原体在淋巴瘤发生中的可能作用。
