Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Med Hypotheses. 2020 Dec;145:110342. doi: 10.1016/j.mehy.2020.110342. Epub 2020 Oct 8.
This study aimed at identifying human neural proteins that can be attacked by cross-reacting SARS-COV-2 antibodies causing Guillain-Barré syndrome. These markers can be used for the diagnosis of Guillain-Barré syndrome (GBS). To achieve this goal, proteins implicated in the development of GBS were retrieved from literature. These human proteins were compared to SARS-COV-2 surface proteins to identify homologous sequences using Blastp. Then, MHC-I and MHC-II epitopes were determined in the homologous sequences and used for further analysis. Similar human and SARS-COV-2 epitopes were docked to the corresponding MHC molecule to compare the binding pattern of human and SARS-COV-2 proteins to the MHC molecule. Neural cell adhesion molecule is the only neural protein that showed homologous sequence to SARS-COV-2 envelope protein. The homologous sequence was part of HLA-A68 and HLA-DQA/HLA-DQB epitopes had a similar binding pattern to SARS-COV-2 envelope protein. Based on these results, the study suggests that NCAM may play a significant role in the immunopathogenesis of GBS. NCAM antibodies can be used as a marker for Guillain-Barré syndrome. However, more experimental studies are needed to prove these results.
本研究旨在鉴定能够被交叉反应的 SARS-CoV-2 抗体攻击的人类神经蛋白，从而导致吉兰-巴雷综合征。这些标志物可用于吉兰-巴雷综合征（GBS）的诊断。为了实现这一目标，从文献中检索了与 GBS 发生相关的蛋白质。使用 Blastp 将这些人类蛋白与 SARS-CoV-2 表面蛋白进行比较，以鉴定同源序列。然后，在同源序列中确定 MHC-I 和 MHC-II 表位，并进行进一步分析。相似的人类和 SARS-CoV-2 表位被对接至相应的 MHC 分子，以比较人类和 SARS-CoV-2 蛋白与 MHC 分子的结合模式。神经细胞黏附分子是唯一显示与 SARS-CoV-2 包膜蛋白同源序列的神经蛋白。该同源序列是 HLA-A68 的一部分，并且 HLA-DQA/HLA-DQB 表位与 SARS-CoV-2 包膜蛋白具有相似的结合模式。基于这些结果，本研究表明 NCAM 可能在 GBS 的免疫发病机制中起重要作用。NCAM 抗体可用作吉兰-巴雷综合征的标志物。然而，需要更多的实验研究来验证这些结果。
