Institute of Sciences of Food Production, National Research Council, Bari, Italy.
Institute of Sciences of Food Production, National Research Council, Bari, Italy.
Waste Manag. 2021 Jan 1;119:215-225. doi: 10.1016/j.wasman.2020.09.048. Epub 2020 Oct 15.
Anaerobic digestion represents an interesting approach to produce biogas from organic waste materials contaminated by mycotoxins. In this study a shotgun metagenomic analysis of lab-scale bioreactors fed with mycotoxin-contaminated silage has been carried out to characterize the evolution of microbial community under the operating conditions and the key enzymatic activities responsible for mycotoxin degradation. The study was conducted at two different level of contamination for fumonisins and aflatoxin B. After 15 days biogas production was not influenced by the presence of mycotoxins. Metagenomic analysis revealed that a high contamination rate of mycotoxins interfere with microbial diversity. Degradation of mycotoxins accounted in about 54% for aflatoxin B and 60% for fumonisins. The degradation activity of fumonisins resulted in the presence of partially hydrolyzed forms in both tested contamination levels. Accordingly, metagenomic functional analysis revealed the presence of two new carboxylesterase genes belonging to D. bacterium and P. bacterium putatively involved in fumonisin degradation.
厌氧消化是一种从受真菌毒素污染的有机废物中生产沼气的有趣方法。本研究采用高通量宏基因组分析方法，对用真菌毒素污染青贮饲料喂养的实验室规模生物反应器进行了分析，以研究在操作条件下微生物群落的演变，以及负责真菌毒素降解的关键酶活性。本研究针对伏马菌素和黄曲霉毒素 B 进行了两个不同污染水平的研究。15 天后，沼气的产生不受真菌毒素的影响。宏基因组分析表明，高浓度的真菌毒素污染会干扰微生物多样性。真菌毒素的降解率约为黄曲霉毒素 B 的 54%和伏马菌素的 60%。伏马菌素的降解活性导致在两种测试的污染水平下都存在部分水解形式。相应地，宏基因组功能分析表明，存在两个属于 D. 菌和 P. 菌的新羧酸酯酶基因，它们可能参与伏马菌素的降解。
