Department of Chemical, Biological, Pharmacological and Environmental Sciences, University of Messina, Messina, Italy,
Department of Chemical, Biological, Pharmacological and Environmental Sciences, University of Messina, Messina, Italy.
Microb Physiol. 2020;30(1-6):50-60. doi: 10.1159/000510874. Epub 2020 Oct 16.
Natural approaches to conventional pharmaceutical treatments for urinary tract infections (UTIs) have focused attention toward reducing the colonization of intestinal Escheri-chia coli reservoirs, the cause of ascending and hematogenous UTIs. In this study, we evaluated the protective effect of xyloglucan and xyloglucan plus gelose on intestinal and urinary epithelia in an in vivo E. coli infection model. Preventative xyloglucan and xyloglucan plus gelose oral treatments were performed by gavage 2 days before E. coli administration and every day until day 7. In vitro, xyloglucan had no effect on bacterial growth, cell morphology, or integrity. The results clearly demonstrated the protective barrier effect of xyloglucan in the bladder and intestine, as evidenced by a reduction in histological changes, neutrophil infiltration, and tight junction permeability in the intestine following E. coli infection. The potential beneficial effect of xyloglucan in preventing UTIs was supported by a reduction of E. coli-positive colony-forming units in the urinary tract. We consider xyloglucan in association with gelose to be an effective oral medical device for the prevention of extraintestinal UTIs.
天然方法治疗尿路感染（UTI）的传统药物治疗方法主要集中在减少肠道大肠杆菌定植，这是上行性和血源性 UTI 的原因。在这项研究中，我们评估了木葡聚糖及其与琼脂糖的混合物对体内大肠杆菌感染模型中肠道和尿路上皮的保护作用。预防性木葡聚糖和木葡聚糖加琼脂糖口服治疗在给予大肠杆菌前 2 天通过灌胃进行，每天一次，直至第 7 天。体外，木葡聚糖对细菌生长、细胞形态或完整性没有影响。结果清楚地表明木葡聚糖在膀胱和肠道中的保护屏障作用，这表现在大肠杆菌感染后肠道的组织学变化、中性粒细胞浸润和紧密连接通透性减少。木葡聚糖在预防 UTI 方面的潜在有益作用得到了减少尿路大肠杆菌阳性集落形成单位的支持。我们认为木葡聚糖与琼脂糖联合使用是预防肠道外 UTI 的有效口服医疗器械。
