Barrett M, Milton A D, Barrett J, Taube D, Bewick M, Parsons V P, Fabre J W
Transplantation. 1987 Aug;44(2):223-7. doi: 10.1097/00007890-198708000-00010.
Twenty needle biopsies from 14 patients were taken at times of renal dysfunction, and frozen sections were stained for class I and class II major histocompatibility complex (MHC) antigen expression using the immunoperoxidase technique and monomorphic mouse monoclonal antibodies. Eight of the 9 biopsies taken during periods of dysfunction attributed to cyclosporine toxicity had normal levels of class II expression. In contrast, 9 of the 10 biopsies taken during episodes of rejection had easily recognized increases in class II expression. In the one case where no definite clinical diagnosis was possible, no class II induction was present. Class I levels were less definitive but tended to be markedly raised in the cases of rejection, and only mildly raised in the cases of nephrotoxicity. Biopsy results can be available within 1 1/2-2 hr. The test is therefore likely to be of value in the correct diagnosis of the cause of renal dysfunction and thereby improve the management of cyclosporine-treated renal transplant patients.
在肾功能不全时，对14例患者进行了20次针吸活检，并使用免疫过氧化物酶技术和单克隆小鼠单克隆抗体对冰冻切片进行I类和II类主要组织相容性复合体（MHC）抗原表达染色。在因环孢素毒性导致功能障碍期间所取的9次活检中，有8次II类表达水平正常。相比之下，在排斥反应发作期间所取的10次活检中，有9次II类表达明显增加。在1例无法明确临床诊断的病例中，未出现II类诱导。I类水平不太明确，但在排斥反应病例中往往明显升高，而在肾毒性病例中仅轻度升高。活检结果可在1个半小时至2小时内获得。因此，该检测可能有助于正确诊断肾功能不全的原因，从而改善接受环孢素治疗的肾移植患者的管理。
