Nast C C
Department of Pathology, Cedars-Sinai Medical Center, Los Angeles, California 90048, USA.
Pediatr Nephrol. 1995;9 Suppl:S56-60. doi: 10.1007/BF00867686.
Fine needle aspiration of renal transplants is a safe effective means of obtaining intragraft material for diagnostic evaluation of transplant dysfunction in the first 3-6 months post engraftment. Aspiration can be performed daily as an outpatient to assess causes of renal insufficiency and response to therapeutic intervention. Aspiration also provides a unique tool for the investigation of pathogenetic mechanisms in acute rejection; samples can be subjected to reverse transcriptase-polymerase chain reaction with quantitation of cytokine gene expression. We have utilized this technique to demonstrate the strong, likely causal, relationship between gamma-interferon and acute rejection. The ability to perform aspirates frequently affords an opportunity to map the cytokine cascade prior to and during episodes of acute rejection. This may identify new areas for therapeutic intervention during the process of rejection.
肾移植细针穿刺抽吸是一种安全有效的方法，可在移植后最初3至6个月内获取移植肾组织材料，用于诊断评估移植肾功能障碍。作为门诊患者，每天都可进行穿刺抽吸，以评估肾功能不全的原因及对治疗干预的反应。穿刺抽吸还为研究急性排斥反应的发病机制提供了一种独特的工具；样本可进行逆转录聚合酶链反应，对细胞因子基因表达进行定量分析。我们利用这项技术证明了γ干扰素与急性排斥反应之间存在强烈的、可能具有因果关系。频繁进行穿刺抽吸的能力提供了一个机会，可在急性排斥反应发作之前和期间描绘细胞因子级联反应。这可能会在排斥反应过程中确定新的治疗干预领域。
