Boland M P, Scaramuzzi R J, Hoskinson R M, Nancarrow C D, Murray J D, Hazelton I G, Sutton R
Vet Rec. 1987 Jun 20;120(25):590-2. doi: 10.1136/vr.120.25.590.
Forty-six adult merino ewes were immunised against oestradiol-17 beta-6 carbomethyloxime:human serum albumin and 48 comparable ewes were used as controls in an experiment to study the effects of gonadotrophin releasing hormone (GnRH) on ovulatory responses after treatment with pregnant mare's serum gonadotrophin (PMSG). All the ewes were treated with progestogen sponges for 14 days and received 1500 iu PMSG on the 12th day. Twenty-four control and 24 immunised ewes received 25 micrograms GnRH 21.5 hours and 23 hours after the sponges were withdrawn. Plasma samples were collected between 17 and 50 hours after the sponges were withdrawn and assayed for luteinising hormone (LH). Immunisation reduced the proportion of ewes which ovulated and their rate of ovulation. Injection of GnRH increased the proportion of immunised ewes ovulating (P less than 0.0005) and their rate of ovulation (P less than 0.0001). More unovulated follicles were observed in immunised ewes regardless of GnRH treatment (P less than 0.0001). The rate of recovery of eggs was reduced after immunisation. Treatment with GnRH produced a surge of LH of equal magnitude in the control and immunised ewes although not as many immunised ewes ovulated.
46只成年美利奴母羊用17β-雌二醇-6-甲氧基肟：人血清白蛋白进行免疫，48只对照母羊用于一项实验，以研究促性腺激素释放激素（GnRH）对孕马血清促性腺激素（PMSG）处理后排卵反应的影响。所有母羊均用孕激素海绵处理14天，并在第12天接受1500国际单位PMSG。24只对照母羊和24只免疫母羊在取出海绵后21.5小时和23小时接受25微克GnRH。在取出海绵后17至50小时之间采集血浆样本，并检测促黄体生成素（LH）。免疫降低了排卵母羊的比例及其排卵率。注射GnRH增加了免疫母羊的排卵比例（P<0.0005）及其排卵率（P<0.0001）。无论是否进行GnRH处理，在免疫母羊中观察到更多未排卵的卵泡（P<0.0001）。免疫后卵子回收率降低。GnRH处理在对照母羊和免疫母羊中产生了幅度相同的LH激增，尽管排卵的免疫母羊数量较少。
