转录组分析在海葵 Nematostella vectensis 中。
Transcriptomic Analysis in the Sea Anemone Nematostella vectensis.
机构信息
Université Côte d'Azur, CNRS, INSERM, Institute for Research on Cancer and Aging, Nice (IRCAN), Nice, France.
Department of Biology and Marine Biology, University of North Carolina at Wilmington, Wilmington, NC, USA.
出版信息
Methods Mol Biol. 2021;2219:231-240. doi: 10.1007/978-1-0716-0974-3_14.
The sea anemone Nematostella vectensis is an emerging research model to study embryonic development and regeneration at the molecular and global transcriptomic level. Transcriptomics analysis is now routinely used to detect differential expression at the genome level. Here we present the latest procedures for isolating high-quality RNA required for next generation sequencing, as well as methods and resources for quantifying transcriptomic data.
海葵 Nematostella vectensis 是一种新兴的研究模式，可以在分子和全转录组水平上研究胚胎发育和再生。转录组分析现在通常用于检测基因组水平上的差异表达。在这里，我们介绍了用于下一代测序的高质量 RNA 分离的最新程序，以及用于定量转录组数据的方法和资源。
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