Department of Cardiovascular Surgery Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Kyoto, Japan.
Regenerative Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Kyoto, Japan.
Ann Thorac Cardiovasc Surg. 2021 Jun 20;27(3):169-175. doi: 10.5761/atcs.oa.20-00133. Epub 2020 Oct 16.
Autologous pericardium is an ideal material for cardiovascular reconstruction including pulmonary artery plasty. Despite the fact that dehydration by ethanol has been used to improve its surgical handling, the effects of the ethanol on mechanical properties of the pericardium have not been previously investigated. The effects of short-duration ethanol dehydration on the mechanical properties of porcine pericardium were evaluated.
Porcine pericardia (n = 3) were separated into three groups: the raw group with no treatments (RAW), the group immersed in 70% ethanol for 10 min (ET group), and the group immersed in 0.6% glutaraldehyde for 10 min (GA). We measured five parameters of mechanical properties as specified in ISO 7198.
ET treatment improved surgical handling as well as GA treatment. There were no significant differences in burst pressure (P = 0.639), suture retention strength (P = 0.529), ultimate tensile strength (UTS; P = 0.486), or Young's modulus (P = 0.408). Only the ultimate strain of the GA group was significantly higher among the three groups (RAW: 33.34% ± 2.02%, ET: 37.48% ± 1.84%, GA: 44.74% ± 2.87%; P = 0.046).
Short-duration ethanol dehydration did not compromise its mechanical properties while maintaining its surgical handling improvements.
自体心包是心血管重建的理想材料，包括肺动脉成形术。尽管乙醇脱水已被用于改善其手术处理，但乙醇对心包机械性能的影响尚未被研究过。本研究评估了短时间乙醇脱水对猪心包机械性能的影响。
将猪心包（n=3）分为三组：未经处理的原始组（RAW）、浸泡在 70%乙醇中 10 分钟的乙醇组（ET）和浸泡在 0.6%戊二醛中 10 分钟的戊二醛组（GA）。我们按照 ISO 7198 的规定测量了五个机械性能参数。
ET 处理改善了手术处理，与 GA 处理一样。在破裂压力（P=0.639）、缝线保持强度（P=0.529）、拉伸强度（UTS；P=0.486）或杨氏模量（P=0.408）方面没有显著差异。只有 GA 组的最终应变显著高于三组（RAW：33.34%±2.02%，ET：37.48%±1.84%，GA：44.74%±2.87%；P=0.046）。
短时间乙醇脱水不会损害其机械性能，同时保持其手术处理的改善。
