基于重组TgSRS2的酶联免疫吸附测定法的开发与评估，用于猫弓形虫感染的血清学诊断。
Development and evaluation of an enzyme-linked immunosorbent assay based on recombinant TgSRS2 for serodiagnosis of Toxoplasma gondii infection in cats.
作者信息
Gao Yang, Guo Huanping, Moumouni Paul Franck Adjou, Liu Mingming, Li Jixu, Efstratiou Artemis, Galon Eloiza May, Ybanez Rochelle Haidee, Masatani Tatsunori, Soma Takehisa, Xuan Xuenan
机构信息
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kogoshima 890-0065, Japan.
出版信息
J Vet Med Sci. 2020 Dec 5;82(11):1662-1665. doi: 10.1292/jvms.20-0231. Epub 2020 Oct 19.
An enzyme-linked immunosorbent assay (ELISA) based on recombinant SAG1-related sequence 2 of Toxoplasma gondii (rTgSRS2) was developed to detect toxoplasmosis in cats. The specificity and sensitivity of rTgSRS2 ELISA were confirmed using a series of serum samples from T. gondii-experimentally infected mice. A total of 76 field samples from cats were examined by the developed ELISA. The rTgSRS2 ELISA showed a good diagnostic performance characterized by high concordance (88.16) and kappa value (0.76) with latex agglutination test (LAT). The sensitivity and specificity of the test were 92.68% and 82.86%, respectively. These results suggest that the ELISA based on rTgSRS2 could be a useful tool for serodiagnosis of T. gondii infection in cats.
开发了一种基于重组弓形虫SAG1相关序列2（rTgSRS2）的酶联免疫吸附测定（ELISA）来检测猫的弓形虫病。使用一系列来自弓形虫实验感染小鼠的血清样本，证实了rTgSRS2 ELISA的特异性和敏感性。通过所开发的ELISA对总共76份猫的野外样本进行了检测。rTgSRS2 ELISA显示出良好的诊断性能，与乳胶凝集试验（LAT）的一致性较高（88.16），kappa值为0.76。该检测的敏感性和特异性分别为92.68%和82.86%。这些结果表明，基于rTgSRS2的ELISA可能是猫弓形虫感染血清学诊断的有用工具。
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