Ybañez Rochelle Haidee D, Kyan Hisako, Nishikawa Yoshifumi
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Okinawa Prefectural Institute of Health and Environment, 17-1 Kanekadan, Uruma-shi, Okinawa 904-2241, Japan.
J Vet Med Sci. 2020 Apr 9;82(4):441-445. doi: 10.1292/jvms.19-0654. Epub 2020 Feb 7.
Toxoplasmosis, caused by Toxoplasma gondii, is one of the most common parasitic diseases worldwide. The GRA7 of T. gondii (TgGRA7) is an essential component of the parasitophorous vacuole (PV) and PV membrane surrounding the tachyzoites and the cyst wall of the bradyzoites. While it has been widely employed as antigen for ELISA, there is only one study that has reported its potential as antigen for immunochromatographic test (ICT) in pigs. There is no study yet documenting its potential for ICT serodiagnosis of T. gondii infection in cats. In this study, we assessed the efficacy of an ICT using TgGRA7 in the detection of Toxoplasma infections in 100 cats and compared the results with iELISAs using TgGRA7 and lysate antigens of T. gondii strains, RH, PLK, and VEG. Our results revealed that TgGRA7-ICT is a reliable test for the diagnosis of anti-T. gondii antibodies in cats, producing comparable results as conventional serological methods. This study is the first report on the use of TgGRA7 as ICT antigen for the serodiagnosis of T. gondii infection in cats.
由刚地弓形虫引起的弓形虫病是全球最常见的寄生虫病之一。刚地弓形虫的致密颗粒蛋白7（TgGRA7）是速殖子周围的寄生泡（PV）和PV膜以及缓殖子囊壁的重要组成部分。虽然它已被广泛用作酶联免疫吸附测定（ELISA）的抗原，但仅有一项研究报道了其作为猪免疫层析试验（ICT）抗原的潜力。尚无研究记录其在猫弓形虫感染ICT血清学诊断中的潜力。在本研究中，我们评估了使用TgGRA7的ICT在检测100只猫弓形虫感染中的效果，并将结果与使用TgGRA7以及弓形虫RH、PLK和VEG株裂解物抗原的间接ELISA结果进行比较。我们的结果显示，TgGRA7-ICT是诊断猫抗弓形虫抗体的可靠检测方法，其结果与传统血清学方法相当。本研究是关于使用TgGRA7作为ICT抗原进行猫弓形虫感染血清学诊断的首次报道。
