Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Changan West Road 620, Xi'an, 710119, China.
Anal Sci. 2021 Jul 10;37(7):1023-1027. doi: 10.2116/analsci.20N023. Epub 2020 Oct 16.
A colorimetric method for detection of acetylcholinesterase (AChE) activity and inhibition was developed using metal organic frameworks (i.e., PCN-222(Fe)) with peroxidase-like activity. The blue tetramethylbenzidine oxidized by PCN-222(Fe) fades due to the reduction by acetylthiocholine chloride produced from AChE catalysis. The detection method shows a linear range of 0.05 - 10 mU mL and a detection limit of 0.03 mU mL AChE. Average recoveries in serum samples varied from 93 to 115% and relative standard deviation (RSD) was lower than 4.9%.
一种使用具有过氧化物酶样活性的金属有机骨架（即 PCN-222(Fe)）检测乙酰胆碱酯酶 (AChE) 活性和抑制的比色法。蓝色四甲基联苯胺被 PCN-222(Fe) 氧化，由于 AChE 催化产生的乙酰硫代胆碱氯化物的还原而褪色。该检测方法的线性范围为 0.05-10 mU mL，检测限为 0.03 mU mL AChE。血清样品中的平均回收率在 93%至 115%之间，相对标准偏差（RSD）低于 4.9%。
