Maecker Holden T, Wang Weiqi, Rosenberg-Hasson Yael, Semelka Richard C, Hickey Joseph, Koran Lorrin M
Stanford University Medical Center, Stanford, CA, USA.
Richard Semelka Consulting, PLLC, Chapel Hill, NC, USA.
Radiol Bras. 2020 Sep-Oct;53(5):306-313. doi: 10.1590/0100-3984.2019.0075.
To determine whether individuals with proposed gadolinium deposition disease (GDD) have elevated serum levels of pro-inflammatory and pro-fibrotic cytokines, and whether specific cytokines are correlated with certain symptoms.
Twenty-four participants recruited between May 2016 and June 2017 met GDD diagnostic criteria. The 64 control subjects provided serum samples before prophylactic flu vaccination. Serum cytokine levels were obtained with Luminex serum cytokine assay using eBiosciences/Affymetrix human 62-plex kits. Wilcoxon rank-sum tests were performed to assess the difference between the median fluorescence intensity values for the participants and the control group. Generalized linear models were built to evaluate the association between each cytokine of interest and selected participant symptoms.
Serum levels of 14 cytokines, including nine pro-inflammatory cytokines, were statistically significantly elevated compared to controls ( ≤ 0.05). Hypotheses regarding pro-fibrotic cytokines and cytokine links to specific symptoms' intensity were not confirmed.
The statistically significantly elevated cytokines may be markers of susceptibility to GDD or agents of symptom induction. These findings suggest that individuals developing symptoms characteristic of GDD after a contrast-assisted magnetic resonance imaging should be studied to investigate whether gadolinium retention and elevated cytokines may be related to their symptoms.
确定疑似钆沉积病（GDD）患者的血清促炎和促纤维化细胞因子水平是否升高，以及特定细胞因子是否与某些症状相关。
2016年5月至2017年6月招募的24名参与者符合GDD诊断标准。64名对照受试者在预防性流感疫苗接种前提供血清样本。使用eBiosciences/Affymetrix人类62种细胞因子检测试剂盒，通过Luminex血清细胞因子检测获得血清细胞因子水平。进行Wilcoxon秩和检验以评估参与者与对照组的中位荧光强度值之间的差异。建立广义线性模型以评估每种感兴趣的细胞因子与选定参与者症状之间的关联。
与对照组相比，14种细胞因子的血清水平在统计学上显著升高，其中包括9种促炎细胞因子（≤0.05）。关于促纤维化细胞因子以及细胞因子与特定症状强度之间联系的假设未得到证实。
统计学上显著升高的细胞因子可能是GDD易感性的标志物或症状诱导因子。这些发现表明，对于在对比增强磁共振成像后出现GDD特征性症状的个体，应进行研究以调查钆潴留和细胞因子升高是否可能与其症状有关。
