Institute of Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia.
Department of Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Osijek, Osijek, Croatia.
Biochem Med (Zagreb). 2020 Oct 15;30(3):030704. doi: 10.11613/BM.2020.030704.
The aim was to evaluate the BD Barricor tubes by comparison with the BD Rapid Serum Tubes (RST) through measuring 25 analytes and monitoring sample stability after 24 hours and 7 days.
Samples of 52 patients from different hospital departments were examined. Blood was collected in BD RST and BD Barricor tubes (Becton, Dickinson and Company, Franklin Lakes, USA). Analytes were measured by Beckman Coulter AU 480 (Beckman Coulter, Brea, USA), Dimension EXL (Siemens Healthcare Diagnostics, Newark, USA) and ARCHITECT i2000SR (Abbott Diagnostics, Lake Forest, USA). Between-tube comparison for each analyte was performed, along with testing analyte stability after storing samples at 4 °C.
BD Barricor tubes showed unacceptable bias compared to BD RST tubes for potassium (K) (- 4.5%) and total protein (4.4%). Analyte stability after 24 hours was acceptable in both tested tubes for most of analytes, except for glucose, aspartate aminotransferase (AST) and lactate dehydrogenase (LD) in BD Barricor and free triiodothyronine in BD RST sample tubes. Analyte stability after 7 days was unacceptable for sodium, K, calcium, creatine kinase isoenzyme MB, AST, LD and troponin I in both samples; additionally for glucose, alkaline phosphatase and albumin in BD Barricor.
All analytes, except K and total protein, can be measured interchangeably in BD RST and BD Barricor tubes, applying the same reference intervals. For most of the analytes, sample re-analysis can be performed in both tubes after 24 hours and 7 days, although BD RST tubes show better 7-day analytes stability over BD Barricor tubes.
本研究旨在通过测量 25 种分析物，并在 24 小时和 7 天后监测样本稳定性，对 BD Barricor 管与 BD Rapid Serum Tubes（RST）进行比较评估。
本研究共检测了来自不同医院科室的 52 名患者的样本。血液分别采集于 BD RST 和 BD Barricor 管（美国贝迪公司）。采用贝克曼库尔特 AU480（贝克曼库尔特公司，美国布雷亚）、西门子 Dimension EXL（美国新泽西州纽瓦克）和雅培诊断 ARCHITECT i2000SR（美国雅培诊断公司，伊利诺伊州莱克福里斯特）检测分析物。对每种分析物进行了管间比较，并测试了在 4°C 下储存样本后分析物的稳定性。
与 BD RST 管相比，BD Barricor 管的钾（K）（-4.5%）和总蛋白（4.4%）存在不可接受的偏差。在两种测试管中，大多数分析物在 24 小时后的稳定性均可接受，除了葡萄糖、天门冬氨酸氨基转移酶（AST）和乳酸脱氢酶（LD）在 BD Barricor 管以及游离三碘甲状腺原氨酸在 BD RST 管。在两种样本中，7 天后钠、K、钙、肌酸激酶同工酶 MB、AST、LD 和肌钙蛋白 I 的稳定性不可接受；此外，BD Barricor 管中葡萄糖、碱性磷酸酶和白蛋白的稳定性也不可接受。
除了 K 和总蛋白，所有分析物都可以在 BD RST 和 BD Barricor 管中互换测量，应用相同的参考区间。对于大多数分析物，在 24 小时和 7 天后，两种管都可以进行样本再分析，尽管与 BD Barricor 管相比，BD RST 管在 7 天内的分析物稳定性更好。
