Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, China.
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China.
Front Immunol. 2020 Sep 25;11:547144. doi: 10.3389/fimmu.2020.547144. eCollection 2020.
Porcine reproductive and respiratory syndrome (PRRS) caused by a single-stranded RNA virus (PRRSV) is a highly infectious respiratory disease and leads to huge economic losses to the swine industry worldwide. To investigate the role of miRNAs in the infection and lung injury induced by PRRSV, the differentially expressed miRNAs (DE-miRs) were isolated from PRRSV-2 infected/mock-infected PAMs of Meishan, Landrace, Pietrain, and Qingping pigs at 9, 36, and 60 hpi. Mir-331-3p was the only common DE-miR in each set of miRNA expression profile at 36 hpi. Mir-210 was one of 7 common DE-miRs between PRRSV infected and mock-infected PAMs of Meishan, Pietrain, and Qingping pigs at 60 hpi. Mir-331-3p/mir-210 could target PRRSV-2 ORF1b, bind and downregulate porcine α/ expression, and inhibit PRRSV-2 replication, respectively. Furthermore, and α could mediate the transcriptional activation of , and . could also upregulate the expression of α by binding to its promoter region. , pEGFP-N1-mir-331-3p could significantly reduce viral replication and pathological changes in PRRSV-2 infected piglets. Taken together, Mir-331-3p/mir-210 have significant roles in the infection and lung injury caused by PRRSV-2, and they may be promising therapeutic targets for PRRS and lung injury/inflammation.
猪繁殖与呼吸综合征（PRRS）由单链 RNA 病毒（PRRSV）引起，是一种高度传染性的呼吸道疾病，给全球养猪业造成巨大经济损失。为研究 miRNA 在 PRRSV 感染和肺损伤中的作用，从梅山、长白、皮特兰和清平猪 PRRSV-2 感染/模拟感染的 PAMs 中分离出差异表达的 miRNA（DE-miRs），分别在 9、36 和 60 hpi 时进行分析。在 36 hpi 时，miR-331-3p 是每种 miRNA 表达谱中唯一的共同 DE-miR。在 60 hpi 时，miR-210 是梅山、皮特兰和清平猪 PRRSV 感染和模拟感染 PAMs 之间的 7 个共同 DE-miR 之一。miR-331-3p/miR-210 可分别靶向 PRRSV-2 ORF1b，结合并下调猪 α/的表达，抑制 PRRSV-2 复制。此外，α可介导 的转录激活，而 可通过结合其启动子区域而上调 的表达。miR-331-3p/pEGFP-N1 可显著降低 PRRSV-2 感染仔猪的病毒复制和病理变化。综上所述，miR-331-3p/miR-210 在 PRRSV-2 感染和肺损伤中具有重要作用，它们可能是 PRRS 和肺损伤/炎症的有希望的治疗靶点。
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