Taiwo Abiola Ezekiel, Madzimbamuto Tafirenyika Nyamayaro, Ojumu Tunde Victor
Department of Chemical Engineering, Cape Peninsula University of Technology, P.O Box 1609, Bellville, 7535, South Africa.
Heliyon. 2020 Oct 5;6(10):e05103. doi: 10.1016/j.heliyon.2020.e05103. eCollection 2020 Oct.
Microbial production of acetoin is eco-friendly and inexpensive when compared with its synthetic methods of production. In the present findings, bioproduction of acetoin in a typical bioreactor was discussed with a view to ascertain the seemingly comparative advantage of bioreactor system over shake flask, and more importantly, to confirm that corn steep liquor can indeed adequately be used as a replacement for other organic nitrogen sources. Taguchi design was statistically used to optimized the fermentation process which resulted in a 3-fold increase in molar yield (83%) corresponding to a six-fold increase in acetoin concentration (63.43 g/L), as compared to a similar study conducted in a shake flask. Although agitation rate was observed to be the most controlling, the bioreactor may underperform at agitation rate greater than 300 rpm. The optimum parameters for acetoin production in this study were 300 rpm agitation, 1.5 slpm aeration, 2 days fermentation time, and pH 6.5. The results show that the commercial production of acetoin can be envisioned using a biological approach that may be of economic advantage.
与合成生产方法相比，微生物生产乙偶姻既环保又廉价。在当前的研究结果中，讨论了在典型生物反应器中乙偶姻的生物生产，目的是确定生物反应器系统相对于摇瓶看似具有的比较优势，更重要的是，确认玉米浆确实可以充分用作其他有机氮源的替代品。采用田口设计对发酵过程进行统计优化，与在摇瓶中进行的类似研究相比，摩尔产率提高了3倍（83%），相应地乙偶姻浓度提高了6倍（63.43 g/L）。尽管观察到搅拌速度是最具控制性的因素，但生物反应器在搅拌速度大于300 rpm时可能表现不佳。本研究中乙偶姻生产的最佳参数为搅拌速度300 rpm、通气量1.5 slpm、发酵时间2天和pH值6.5。结果表明，使用可能具有经济优势的生物方法来设想乙偶姻的商业化生产是可行的。
