Department of Pharmacology, Nanjing Medical University, Nanjing, 211166, Jiangsu, China.
Department of Pharmacy, Sir Run Run Hospital, Nanjing Medical University, Nanjing, 211100, Jiangsu, China.
Mikrochim Acta. 2020 Oct 19;187(11):614. doi: 10.1007/s00604-020-04583-2.
A novel molecularly imprinted ratiometric fluorescent probe was fabricated by simple sol-gel polymerization for selective and sensitive assay of C-type natriuretic peptide (CNP) in biosamples. Both the nitrobenzoxadiazole (NBD) and carbon dots (CDs) were located on the surface of silica, used as the detection signal and reference signal, respectively. For the turn-on-based probe, the fluorescence intensity of NBD could be quantitatively enhanced by CNP based on the strategy of photo-induced electron transfer (PET), while the fluorescence of CDs remained unchanged. The obtained probe exhibited excellent recognition selectivity and fast kinetics to CNP templates, and also showed good stability. The linear range of CNP determination was 5-80 pg mL with a low detection limit of 2.87 pg mL. Finally, the probe was successfully applied to determine CNP in human serum samples and attained high recoveries between 97.3 and 104% with precisions below 4.7%. The result indicates that the proposed method has promising potential for the assay of trace peptides in complex matrices. Schematic illustration for the formation and determination mechanism of the probe.
一种新型的分子印迹比率荧光探针通过简单的溶胶-凝胶聚合制备，用于生物样品中 C 型利钠肽（CNP）的选择性和灵敏测定。硝基苯并二唑（NBD）和碳点（CDs）都位于硅烷表面，分别用作检测信号和参考信号。对于基于开启的探针，基于光诱导电子转移（PET）的策略，NBD 的荧光强度可以被 CNP 定量增强，而 CDs 的荧光保持不变。所得到的探针对 CNP 模板表现出优异的识别选择性和快速动力学，并且还表现出良好的稳定性。CNP 测定的线性范围为 5-80 pg mL，检测限低至 2.87 pg mL。最后，该探针成功应用于人血清样品中 CNP 的测定，回收率在 97.3%至 104%之间，精密度低于 4.7%。结果表明，该方法在复杂基质中痕量肽的测定中具有广阔的应用前景。探针的形成和测定机制示意图。
