Faculty of Medical Sciences, Department of Medical Physics, Tarbiat Modares University, Tehran, Iran.
Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Int J Radiat Biol. 2020 Dec;96(12):1614-1627. doi: 10.1080/09553002.2020.1838657. Epub 2020 Nov 9.
This study purposed to evaluate the efficacy of brachytherapy with the modality of ultrasonic hyperthermia in the presence of gold nanoparticles (GNPs) on an ocular retinoblastoma tumor in an animal model of the rabbit.
A retinoblastoma tumor was induced by the injection of the human cell line of Y79 in rabbit eyes ( = 41). After two weeks, tumor size reached a diameter of about 5-7 mm. Seven groups were involved: control, GNPs injection, hyperthermia, hyperthermia with GNPs injection, brachytherapy with I-125, a combination of hyperthermia and brachytherapy, and a combination of brachytherapy, hyperthermia and, GNPs. The tumor area was measured using B-mode ultrasound images on the zero-day and at the end of the third week. The groups were evaluated for a histopathological study of tumor necrosis.
There was a significant difference between the relative area changes of tumor in the combination group with the other study groups ( < .05). The results of histopathologic studies confirmed the necrosis of living retinoblastoma cells.
Combination therapy of brachytherapy and hyperthermia with GNPs reduces the relative size of the tumor. This method increases the necrosis percentage of retinoblastoma and significantly reduces the retinoblastoma mass in the rabbit eyes.
本研究旨在评估在金纳米颗粒（GNPs）存在的情况下，超声热疗模式下近距离放射治疗对兔眼视网膜母细胞瘤肿瘤的疗效。
通过向兔眼内注射人 Y79 细胞系诱导视网膜母细胞瘤肿瘤（ = 41）。两周后，肿瘤大小达到约 5-7 毫米。共涉及 7 个组：对照组、GNPs 注射组、热疗组、GNPs 联合热疗组、近距离放射治疗 I-125 组、热疗联合近距离放射治疗组、热疗联合近距离放射治疗和 GNPs 组。在第 0 天和第 3 周末使用 B 型超声图像测量肿瘤区域。对肿瘤坏死的组织病理学研究评估各组。
联合组与其他研究组的肿瘤相对面积变化有显著差异（ < .05）。组织病理学研究结果证实了活视网膜母细胞瘤细胞的坏死。
近距离放射治疗联合 GNPs 热疗可减少肿瘤的相对大小。这种方法增加了视网膜母细胞瘤的坏死百分比，显著减少了兔眼内的视网膜母细胞瘤质量。
