Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Department of pharmaceutical analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
J Fluoresc. 2021 Jan;31(1):85-90. doi: 10.1007/s10895-020-02638-4. Epub 2020 Oct 19.
In this work, a novel ecofriendly optical nanosensor for detection of Eplerenone (EPL) in biological samples was reported. Highly luminescent water-soluble nitrogen and sulfur doped carbon quantum dots (N, S-CQDs) have been prepared successfully. The synthesis was based on the reaction of thiosemicarbazide (TS) as source of N and S and citric acid (CA) as source of carbon in one-step aqueous base reflux treatment. The produced N, S-CQDs have a small particle size in the range of 4.7 nm with a high quantum yield (58.5%) and high emission intensity at 446 nm under excitation wavelength of 370 nm. The unique properties of N, S-CQDs make them useful tool as a nano fluorescent probe for sensitive determination of EPL. EPL has been found to decrease the fluorescence of S, N-CDs significantly through static quenching according to the Stern - Volmer plot. The decreased intensity of S, N-CDs fluorescence was proportional to EPL in the 0.2-3.0 μM range. The limit of detection and quantitation were 0.05 and 0.15 μM, respectively. The assay of EPL by this approach was successfully done in drug formulations and in spiked human serum samples.
本工作报道了一种用于检测生物样品中依普利酮（EPL）的新型环保光学纳米传感器。成功制备了高发光水溶性氮硫掺杂碳量子点（N、S-CQDs）。该合成方法基于一步水相碱性回流处理中，以硫代半卡巴肼（TS）作为 N 和 S 的来源，以柠檬酸（CA）作为碳的来源进行反应。所制备的 N、S-CQDs 的粒径在 4.7nm 范围内，量子产率（58.5%）高，在 370nm 激发波长下在 446nm 处发射强度高。N、S-CQDs 的独特性质使其成为一种有用的纳米荧光探针，可用于 EPL 的灵敏测定。根据 Stern-Volmer 图，EPL 通过静态猝灭显著降低了 S、N-CDs 的荧光。S、N-CDs 荧光强度的降低与 0.2-3.0μM 范围内的 EPL 成正比。检测限和定量限分别为 0.05 和 0.15μM。该方法成功地用于药物制剂和加标人血清样品中的 EPL 测定。
