Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Luminescence. 2021 Sep;36(6):1435-1443. doi: 10.1002/bio.4083. Epub 2021 Jun 3.
Water-soluble and highly stable N,S-doped CQDs (N,S-CQDs) were synthesized using a low-cost strategy with citric acid and thiosemicarbazide in one step for use as a fluorescent nanosensor. The achieved N,S-CQDs produced strong emission at 446 nm upon excitation at 370 nm and a high quantum yield of 58.5%. The quenching effect on the prepared N,S-CQDs was utilized for determination of trimetazidine (TMZ) spectrofluorimetrically over a wide linear range 0.04-0.5 μM (0.0106-0.133 μg ml ) and a low limit of detection of 0.01 μM (0.002 μg ml ). Furthermore, CDs were used as a simple and rapid fluorescent probe to determine TMZ in its pharmaceutical formulations as well as in human plasma. The method was tested in compliance with International Council for Harmonisation guidelines. The results obtained were compared statistically with those given for a reported method showing no significant variation regards accuracy and precision.
采用一步法，以柠檬酸和氨基硫脲为原料，合成了水溶性和高稳定性的 N，S 掺杂碳量子点（N，S-CQDs），用作荧光纳米传感器。所得到的 N，S-CQDs 在 370nm 激发下于 446nm 处产生强发射，量子产率高达 58.5%。利用制备的 N，S-CQDs 的猝灭效应，通过分光荧光法在 0.04-0.5μM（0.0106-0.133μg·ml-1）的较宽线性范围内和低检测限 0.01μM（0.002μg·ml-1）下测定曲美他嗪（TMZ）。此外，将 CDs 用作简单快速的荧光探针，用于测定其药物制剂以及人血浆中的 TMZ。该方法符合国际协调理事会的指导原则。所得结果与报道方法的结果进行了统计学比较，在准确性和精密度方面没有显著差异。
