Beckman W C, Jacokes A L, Camps J L, Cook R L, Siegal G P
Am J Pathol. 1987 Sep;128(3):566-72.
The R3327-H model of prostatic adenocarcinoma was employed for the study of the cellular changes that occur during induction, regression, and recurrence of prostate cancer after endocrine therapy. The present study was designed to compare the glandular and stromal elements of the relapse phase with the histologically distinct early and intermediate phases of tumor progression. Morphometric analysis revealed significant differences between all three groups in the percentages of total tumor occupied by the epithelial component. At all three time periods, high-power inspection of autoradiograms prepared after incubation of the tissues with radioactive dihydrotestosterone revealed large cells in the stroma, especially in the intermediate phase. Immunohistochemistry further revealed evidence of invasion across the prostatic acinar basement membranes by similar cells. These studies lead the authors to postulate a mechanism by which hormone-independent cells in the epithelium repopulate the stroma, causing a recapitulation of the original morphology of the tumor in the postremission period. They propose that prostate tumor response to estrogen therapy can be operationally defined in three phases: involution, rebound, and relapse. They infer that further knowledge of the timing of these phases may permit early selective use of specific therapeutic strategies which will be able to balance the clinical risk with the known behavior of the neoplasm during progression of the disease.
采用前列腺腺癌R3327-H模型研究内分泌治疗后前列腺癌诱导、消退和复发过程中发生的细胞变化。本研究旨在比较复发期的腺体和间质成分与组织学上不同的肿瘤进展早期和中期。形态计量分析显示，三组之间上皮成分占总肿瘤的百分比存在显著差异。在所有三个时间段，用放射性双氢睾酮孵育组织后制备的放射自显影片的高倍检查显示，间质中有大细胞，尤其是在中期。免疫组织化学进一步显示，类似细胞侵袭前列腺腺泡基底膜。这些研究使作者推测出一种机制，即上皮中的激素非依赖性细胞重新填充间质，导致缓解期肿瘤原始形态的重现。他们提出，前列腺肿瘤对雌激素治疗的反应可在三个阶段进行操作性定义：退化、反弹和复发。他们推断，对这些阶段时间的进一步了解可能允许早期选择性使用特定的治疗策略，这些策略将能够在疾病进展过程中平衡临床风险与肿瘤的已知行为。
