Zhvirblene A A, Noreĭka R K, Sazykin A Iu
Antibiot Med Biotekhnol. 1987 Apr;32(4):279-82.
Two modifications of solid phase enzyme immunoassay (EIAA) for insulin with the use of beta-lactamase from B. licheniformis 749/c and horse radish peroxidase as the markers were developed. beta-Lactamase and peroxidase conjugates with insulin were prepared with the glutaric aldehyde and periodate methods respectively. Both the conjugates were stable and preserved high immunospecific and enzymatic activity. Sensitivity of EIAA with the use of the beta-lactamase or peroxidase as the markers was the same and amounted to 15 ng/ml of insulin. However, with the use of the beta-lactamase in EIAA not only spectrophotometric recording but also visual registration of the results was possible.
开发了两种使用地衣芽孢杆菌749/c的β-内酰胺酶和辣根过氧化物酶作为标记物的固相酶免疫测定法（EIAA）来检测胰岛素。分别采用戊二醛法和高碘酸盐法制备了β-内酰胺酶和过氧化物酶与胰岛素的缀合物。两种缀合物均稳定，保持了高免疫特异性和酶活性。使用β-内酰胺酶或过氧化物酶作为标记物的EIAA灵敏度相同，均为15 ng/ml胰岛素。然而，在EIAA中使用β-内酰胺酶时，不仅可以进行分光光度记录，还可以进行结果的目视记录。
