Levi M I, Shimko T A, Sazykin A Iu, Navashin S M
Antibiot Med Biotekhnol. 1985 Sep;30(9):662-5.
beta-Lactamase from Bacillus licheniformis 749/c was used as a marker for ELISA. Conjugates of the beta-lactamase with the capsule antigen of the plague causative agent and with the monoclonal antibodies to it were prepared by glutaric aldehyde "linking". The conjugates preserved high immunospecific and beta-lactamase activity. High sensitivity of the modification of ELISA allowed detecting up to 8 ng/ml of the capsule antigen of the plague causative agent. The enzymatic activity of the beta-lactamase was determined microiodometrically which provided visual recording of the results of ELISA.
地衣芽孢杆菌749/c的β-内酰胺酶被用作酶联免疫吸附测定（ELISA）的标记物。通过戊二醛“连接”制备了β-内酰胺酶与鼠疫病原体荚膜抗原及其单克隆抗体的缀合物。这些缀合物保留了高免疫特异性和β-内酰胺酶活性。ELISA改良法的高灵敏度能够检测到高达8纳克/毫升的鼠疫病原体荚膜抗原。β-内酰胺酶的酶活性通过微量碘量法测定，这使得ELISA结果能够进行可视化记录。
