Hrdlička Vojtěch, Barek Jiří, Navrátil Tomáš
J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Dolejškova 2155/3, 182 23, Prague 8, Czech Republic; Charles University, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Hlavova 2030/8, 128 43, Prague 2, Czech Republic.
Charles University, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Hlavova 2030/8, 128 43, Prague 2, Czech Republic.
Talanta. 2021 Jan 1;221:121594. doi: 10.1016/j.talanta.2020.121594. Epub 2020 Sep 8.
Novel method for the determination of a tumor marker homovanillic acid (HVA) in human urine was developed. Combination of hollow fiber - based liquid-phase microextraction (HF-LPME) and differential pulse voltammetry (DPV) at a cathodically pre-treated boron doped diamond electrode (BDDE) was applied for these purposes. Optimum conditions were: butyl benzoate as supported liquid membrane (SLM) formed on polypropylene HF, 0.1 mol L HCl as donor phase, 0.1 mol L sodium phosphate buffer of pH 6 as acceptor phase, and 30 min extraction time. HF-LPME-DPV concentration dependence was linear in the range from 1.2 to 100 μmol L. Limits of quantification (LOQ) and detection (LOD) were 1.2 and 0.4 μmol L, respectively. The applicability of the developed method was verified by analysis of human urine. Standard addition method was used, found HVA concentration was 13.5 ± 1.3 μmol L, RSD = 9.3% (n=5).
开发了一种测定人尿中肿瘤标志物高香草酸（HVA）的新方法。为此，采用了基于中空纤维的液相微萃取（HF-LPME）与在阴极预处理的硼掺杂金刚石电极（BDDE）上的差分脉冲伏安法（DPV）相结合的方法。最佳条件为：以苯甲酸丁酯作为在聚丙烯中空纤维上形成的支撑液膜（SLM），0.1 mol L盐酸作为供体相，pH 6的0.1 mol L磷酸钠缓冲液作为受体相，萃取时间为30分钟。HF-LPME-DPV的浓度依赖性在1.2至100 μmol L范围内呈线性。定量限（LOQ）和检测限（LOD）分别为1.2和0.4 μmol L。通过对人尿的分析验证了所开发方法的适用性。采用标准加入法，测得HVA浓度为13.5±1.3 μmol L，相对标准偏差（RSD）=9.3%（n = 5）。
