Department of Chemistry, University of Mazandaran, Babolsar, Iran.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Jul 1;900:85-8. doi: 10.1016/j.jchromb.2012.05.022. Epub 2012 May 24.
The applicability of hollow fiber liquid phase microextraction (HF-LPME) was evaluated for extraction and preconcentration of apigenin prior to its determination by HPLC. Different parameters affecting the HF-LPME recovery such as nature of organic solvent, pH of donor and acceptor phases, extraction time, stirring speed, salt addition were optimized. Under optimum conditions (1-octanol as organic solvent, pH of the donor phase=3 and pH of acceptor phase=11.5, extraction time of 75 min, stirring speed of 1000 rpm) limit of detection (LOD) of 0.1 ng/mL, linear range of 0.5-300 ng/mL and correlation of determination (R(2)) of 0.9956 were obtained. The relative intra and inter-day standard deviations (RSD%) based on five replicate measurement were 3.5% and 10.7% respectively. Enrichment factor of 315 and recovery 85% were achieved. Finally, the applicability of the proposed method was evaluated by extraction and determination of apigenin in urine sample after consumption of Satureja sahendica Bornm. which is a native medicinal plant from Iran. Concentration of apigenin in urine sample was found to be 6.20 ng/mL.
采用中空纤维液相微萃取（HF-LPME），在高效液相色谱法测定之前，对芹菜素进行萃取和预浓缩。优化了影响 HF-LPME 回收率的不同参数，如有机溶剂的性质、供体和受体相的 pH 值、萃取时间、搅拌速度、加盐等。在最佳条件下（1-辛醇为有机溶剂，供体相 pH 值为 3，受体相 pH 值为 11.5，萃取时间为 75 分钟，搅拌速度为 1000rpm），检测限（LOD）为 0.1ng/mL，线性范围为 0.5-300ng/mL，相关系数（R²）为 0.9956。基于五次重复测量的相对日内和日间标准偏差（RSD%）分别为 3.5%和 10.7%。实现了 315 的富集因子和 85%的回收率。最后，通过对伊朗本土药用植物 Satureja sahendica Bornm 摄入后尿液中芹菜素的提取和测定，评估了所提出方法的适用性。尿液样品中芹菜素的浓度为 6.20ng/mL。
