Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA.
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, USA.
Fluids Barriers CNS. 2020 Oct 19;17(1):64. doi: 10.1186/s12987-020-00222-3.
In 2012, we provided the first published evidence that human pluripotent stem cells could be differentiated to cells exhibiting markers and phenotypes characteristic of the blood-brain barrier (BBB). In the ensuing years, the initial protocols have been refined, and the research community has identified both positive and negative attributes of this stem cell-based BBB model system. Here, we give our perspective on the current status of these models and their use in the BBB community, as well as highlight key attributes that would benefit from improvement moving forward.
2012 年，我们首次提供了人类多能干细胞可分化为表现出血脑屏障（BBB）特征性标志物和表型的细胞的证据。在随后的几年中，初始方案得到了改进，研究人员也确定了这种基于干细胞的 BBB 模型系统的优缺点。在这里，我们介绍了这些模型的现状及其在 BBB 研究领域的应用，并强调了在未来需要改进的关键属性。
