HiLIFE, Neuroscience Center, University of Helsinki, Haartmaninkatu 8, 00290, Helsinki, Finland.
Aalto University, Micronova, Tietotie 3, 02150, Espoo, Finland.
Sci Rep. 2020 Oct 19;10(1):17661. doi: 10.1038/s41598-020-74535-6.
Different types of carbon materials are biocompatible with neural cells and can promote maturation. The mechanism of this effect is not clear. Here we have tested the capacity of a carbon material composed of amorphous sp carbon backbone, embedded with a percolating network of sp carbon domains to sustain neuronal cultures. We found that cortical neurons survive and develop faster on this novel carbon material. After 3 days in culture, there is a precocious increase in the frequency of neuronal activity and in the expression of maturation marker KCC2 on carbon films as compared to a commonly used glass surface. Accelerated development is accompanied by a dramatic increase in neuronal dendrite arborization. The mechanism for the precocious maturation involves the activation of intracellular calcium oscillations by the carbon material already after 1 day in culture. Carbon-induced oscillations are independent of network activity and reflect intrinsic spontaneous activation of developing neurons. Thus, these results reveal a novel mechanism for carbon material-induced neuronal survival and maturation.
不同类型的碳材料与神经细胞具有生物相容性，并能促进其成熟。其作用机制尚不清楚。在此，我们测试了由无定形 sp 碳骨架组成并嵌入 sp 碳畴渗透网络的碳材料在维持神经元培养方面的能力。我们发现皮质神经元在这种新型碳材料上存活和发育得更快。与常用的玻璃表面相比，在培养 3 天后，碳膜上神经元活动的频率和成熟标志物 KCC2 的表达会提前增加。快速发育伴随着神经元树突分支的急剧增加。早熟成熟的机制涉及到细胞内钙振荡的激活，这一过程在培养 1 天后就已经发生。碳诱导的振荡独立于网络活动，反映了发育中的神经元内在的自发激活。因此，这些结果揭示了碳材料诱导神经元存活和成熟的一种新机制。
