Jones A F, Lunec J
Department of Biochemistry, Selly Oak Hospital, Birmingham, UK.
Br J Cancer Suppl. 1987 Jun;8:60-5.
Evidence for free radical involvement in pathological processes is often indirect and frequently depends upon the detection of characteristic changes in tissue constituents, particularly polyunsaturated lipids. Free radical attack on protein leads to specific oxidative changes in their constituent amino acids, principally cysteine, tryptophan and tyrosine. This is associated with the induction of characteristic fluorescence (excitation 360 nm, emission 454 nm) and protein aggregation. This observation leads to a suitable assay for studying protein oxidation, and such fluorescent proteins may be relevant in the pathogenesis of diabetic microangiopathy and rheumatoid arthritis.
自由基参与病理过程的证据往往是间接的，通常依赖于对组织成分，特别是多不饱和脂质特征性变化的检测。自由基对蛋白质的攻击会导致其组成氨基酸发生特定的氧化变化，主要是半胱氨酸、色氨酸和酪氨酸。这与特征性荧光（激发波长360nm，发射波长454nm）的诱导和蛋白质聚集有关。这一观察结果为研究蛋白质氧化提供了一种合适的检测方法，并且这种荧光蛋白可能与糖尿病微血管病变和类风湿性关节炎的发病机制有关。
