Laboratory of Ecology and Conservation, Federal University of Fronteira Sul, Erechim Campus, Brazil, ERS 135 - Km 72, nº 200, Erechim, RS, Brazil.
Environ Toxicol Pharmacol. 2021 Jan;81:103516. doi: 10.1016/j.etap.2020.103516. Epub 2020 Oct 17.
Genotoxicity studies have revealed that pesticides bind to genetic material in non-target vertebrates, thereby impairing the genetic integrity of these animals. The main objective of this study was to determine the genotoxic damage in erythrocytes of two native South American amphibian Physalaemus cuvieri and Physalaemus gracilis, both species exposed to a glyphosate-based herbicide. We evaluated the presence of micronuclei (MN) and erythrocyte nuclear abnormalities (ENA) as biomarkers for potential genotoxic compounds. Tadpoles were exposed to doses permitted by Brazilian legislation and concentrations found naturally in Brazilian and Argentinian waters (500, 700 and 1000 μg/L). Glyphosate-based herbicide caused micronuclei formation and several types of erythrocyte nuclear abnormalities in both Physalaemus species. The total frequency of MN and ENA demonstrated the occurrence of cell damage at all tested concentrations. Glyphosate herbicide can be considered a genotoxic that may impact the genetic integrity of native populations of P. cuvieri and P. gracilis.
遗传毒性研究表明，农药会与非目标脊椎动物的遗传物质结合，从而损害这些动物的遗传完整性。本研究的主要目的是确定两种本地的南美洲两栖动物 Physalaemus cuvieri 和 Physalaemus gracilis 的红细胞中的遗传毒性损伤，这两种物种都暴露于草甘膦基除草剂中。我们评估了微核（MN）和红细胞核异常（ENA）的存在，作为潜在遗传毒性化合物的生物标志物。蝌蚪暴露于巴西法规允许的剂量和巴西和阿根廷水域中自然存在的浓度（500、700 和 1000 μg/L）。草甘膦基除草剂导致两种 Physalaemus 物种形成微核和几种类型的红细胞核异常。MN 和 ENA 的总频率表明在所有测试浓度下都发生了细胞损伤。草甘膦除草剂可以被认为是一种遗传毒性物质，可能会影响 P. cuvieri 和 P. gracilis 等本地种群的遗传完整性。
