Hussain Abid, Ali Muhammad Waqar, AlJabr Ahmed Mohammed, Al-Kahtani Saad Naser
Laboratory of Bio-Control and Molecular Biology, Department of Arid Land Agriculture, College of Agricultural and Food Sciences, King Faisal University, Hofuf 31982, Saudi Arabia.
Institute of Research and Consultancy, King Faisal University, Hofuf 31982, Saudi Arabia.
J Fungi (Basel). 2020 Oct 18;6(4):232. doi: 10.3390/jof6040232.
Natural pathogen pressure is an important factor that shapes the host immune defense mechanism. The current study primarily aimed to explore the molecular basis of the natural immune defense mechanism of a sporadic pest, , during swarming by constructing cDNA libraries of the female mid-gut, male mid-gut, testes, and ovaries. The Illumina HiSeq platform generated an average of 7.9 G, 11.77 G, 10.07 G, and 10.07 G bases of outputs from the male mid-gut, female mid-gut, testes, and ovaries and libraries, respectively. The transcriptome of two-spotted field crickets was assembled into 233,172 UniGenes, which yielded approximately 163.58 million reads. On the other hand, there were 43,055 genes in common that were shared among all the biological samples. Gene Ontology analysis successfully annotated 492 immune-related genes, which comprised mainly Pattern Recognition Receptors (62 genes), Signal modulators (57 genes), Signal transduction (214 genes), Effectors (36 genes), and another immune-related 123 genes. In summary, the identified wide range of immune-related genes from indicates the existence of a sophisticated and specialized broad spectrum immune mechanism against invading pathogens, which provides, for the first time, insights into the molecular mechanism of disease resistance among two-spotted field crickets.
自然病原体压力是塑造宿主免疫防御机制的一个重要因素。当前研究主要旨在通过构建雌性中肠、雄性中肠、睾丸和卵巢的cDNA文库，探索一种散发性害虫在群居期间自然免疫防御机制的分子基础。Illumina HiSeq平台分别从雄性中肠、雌性中肠、睾丸和卵巢及文库中平均产生了7.9 G、11.77 G、10.07 G和10.07 G碱基的输出量。二斑蟋蟀的转录组被组装成233,172个单基因，产生了约1.6358亿条读数。另一方面，所有生物样本共有43,055个共同基因。基因本体分析成功注释了492个免疫相关基因，主要包括模式识别受体（62个基因）、信号调节剂（57个基因）、信号转导（214个基因）、效应器（36个基因）以及另外123个免疫相关基因。总之，从[此处原文缺失具体昆虫名称]中鉴定出的广泛免疫相关基因表明存在一种针对入侵病原体的复杂且专门的广谱免疫机制，这首次为二斑蟋蟀的抗病分子机制提供了见解。
