Mochizuki Takako, Sakamoto Mika, Tanizawa Yasuhiro, Seike Hitomi, Zhu Zhen, Zhou Yi Jun, Fukumura Keisuke, Nagata Shinji, Nakamura Yasukazu
National Institute of Genetics, Research Organization of Information and Systems, 1111 Yata, Mishima, Shizuoka 411-8540, Japan.
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
Insects. 2023 Jan 25;14(2):121. doi: 10.3390/insects14020121.
Genome annotation is critically important data that can support research. Draft genome annotations cover representative genes; however, they often do not include genes that are expressed only in limited tissues and stages, or genes with low expression levels. Neuropeptides are responsible for regulation of various physiological and biological processes. A recent study disclosed the genome draft of the two-spotted cricket , which was utilized to understand the intriguing physiology and biology of crickets. Thus far, only two of the nine reported neuropeptides in were annotated in the draft genome. Even though de novo assembly using transcriptomic analyses can comprehensively identify neuropeptides, this method does not follow those annotations on the genome locus. In this study, we performed the annotations based on the reference mapping, de novo transcriptome assembly, and manual curation. Consequently, we identified 41 neuropeptides out of 43 neuropeptides, which were reported in the insects. Further, 32 of the identified neuropeptides on the genomic loci in were annotated. The present annotation methods can be applicable for the neuropeptide annotation of other insects. Furthermore, the methods will help to generate useful infrastructures for studies relevant to neuropeptides.
基因组注释是支持研究的至关重要的数据。基因组注释草图涵盖了代表性基因；然而，它们通常不包括仅在有限组织和阶段表达的基因，或低表达水平的基因。神经肽负责调节各种生理和生物学过程。最近的一项研究公布了双斑蟋蟀的基因组草图，该草图被用于了解蟋蟀有趣的生理学和生物学特性。到目前为止，在已公布的基因组草图中，仅对九种已报道的神经肽中的两种进行了注释。尽管使用转录组分析进行从头组装可以全面识别神经肽，但这种方法并不遵循基因组位点上的那些注释。在本研究中，我们基于参考映射、从头转录组组装和人工编辑进行了注释。因此，我们从昆虫中已报道的43种神经肽中鉴定出了41种。此外，在已公布的基因组位点上鉴定出的32种神经肽也得到了注释。目前的注释方法可应用于其他昆虫的神经肽注释。此外，这些方法将有助于为与神经肽相关的研究生成有用的基础设施。
