Stranieri Angelica, Scavone Donatella, Paltrinieri Saverio, Giordano Alessia, Bonsembiante Federico, Ferro Silvia, Gelain Maria Elena, Meazzi Sara, Lauzi Stefania
Department of Veterinary Medicine, University of Milan, 26900 Lodi, Italy.
Veterinary Teaching Hospital, University of Milano, 26900 Lodi, Italy.
Pathogens. 2020 Oct 18;9(10):852. doi: 10.3390/pathogens9100852.
Histology, immunohistochemistry (IHC), and reverse transcription polymerase chain reaction (RT-PCR) have been used to diagnose feline infectious peritonitis (FIP), but no information regarding the comparison of their diagnostic performances on the same organ is available. The aims of this study were to determine the concordance among these tests and to evaluate which combination of tests and organs can be used in vivo. Histology, IHC, and nested RT-PCR (RT-nPCR) for feline coronavirus (FCoV) were performed on spleen, liver, mesenteric lymph node, kidney, large and small intestine, and lung from 14 FIP and 12 non-FIP cats. Sensitivity, specificity, predictive values, likelihood ratios, and concordance were calculated. IHC and RT-nPCR had the highest concordance in lung and liver, histology and IHC in the other organs. The sensitivity of histology, IHC, and RT-nPCR on the different organs ranged from 41.7 to 76.9%, 46.2 to 76.9%, and 64.3 to 85.7%, respectively, and their specificity ranged from 83.3 to 100.0%, 100% and 83.3 to 100.0%. Therefore, IHC is recommended when histology is consistent with FIP. If RT-nPCR is performed as the first diagnostic approach, results should always be confirmed with IHC. Lung or liver provide accurate information regardless of the method, while IHC is preferred to RT-nPCR to confirm FIP in the kidney or intestine.
组织学、免疫组织化学（IHC）和逆转录聚合酶链反应（RT-PCR）已被用于诊断猫传染性腹膜炎（FIP），但尚无关于它们在同一器官上诊断性能比较的信息。本研究的目的是确定这些检测方法之间的一致性，并评估哪些检测方法与器官的组合可用于活体检测。对14只患FIP的猫和12只未患FIP的猫的脾脏、肝脏、肠系膜淋巴结、肾脏、大小肠及肺组织进行了FCoV的组织学、IHC及巢式RT-PCR（RT-nPCR）检测。计算了敏感性、特异性、预测值、似然比及一致性。IHC与RT-nPCR在肺和肝脏中的一致性最高，而在其他器官中组织学与IHC的一致性最高。组织学、IHC及RT-nPCR在不同器官上的敏感性分别为41.7%至76.9%、46.2%至76.9%及64.3%至85.7%，其特异性分别为83.3%至100.0%、100%及83.3%至100.0%。因此，当组织学结果与FIP一致时，推荐使用IHC。如果将RT-nPCR作为首选诊断方法，其结果应始终通过IHC进行确认。无论采用何种方法，肺或肝脏都能提供准确信息，而在肾脏或肠道中确认FIP时，IHC优于RT-nPCR。
