Sihvola M, Hurme M
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Cell Immunol. 1987 Oct 1;109(1):115-22. doi: 10.1016/0008-8749(87)90297-8.
Spleen cells from irradiated, bone marrow-reconstituted mice were tested for their ability to mediate antibody-dependent cellular cytotoxicity against P815 target (ADCC-P815), ADCC against sheep red blood cells (ADCC-SRBC), and natural killer (NK) activity judged as YAC-1 lysis at different times after bone marrow reconstitution. Donor-derived ADCC-P815 effectors were found to appear in the spleens 10-12 days after bone marrow reconstitution simultaneously with the appearance of donor-derived NK cells. NK cells recently derived from bone marrow are known to express the Thy-1 antigen; the phenotype of the "early" ADCC-P815 effectors was found to be the same as that of NK cells, i.e., Thy-1+, asialo-GM1+. These data suggest that ADCC-P815 effector cells belong to the NK cell population. ADCC-SRBC, in contrast to ADCC-P815 and NK activity, was already high on Day 7 after bone marrow reconstitution. However, it was mediated partly by recipient-derived effectors. ADCC-SRBC effectors were characterized to be different from ADCC-P815 effectors.
对经辐照并进行骨髓重建的小鼠的脾细胞进行检测，以评估其在骨髓重建后不同时间介导针对P815靶细胞的抗体依赖性细胞毒性（ADCC-P815）、针对绵羊红细胞的ADCC（ADCC-SRBC）以及作为YAC-1细胞裂解判定的自然杀伤（NK）活性的能力。发现供体来源的ADCC-P815效应细胞在骨髓重建后10 - 12天出现在脾脏中，同时供体来源的NK细胞也出现。已知最近源自骨髓的NK细胞表达Thy-1抗原；发现“早期”ADCC-P815效应细胞的表型与NK细胞相同，即Thy-1 +、无唾液酸GM1 +。这些数据表明ADCC-P815效应细胞属于NK细胞群体。与ADCC-P815和NK活性相反，ADCC-SRBC在骨髓重建后第7天就已经很高。然而，它部分由受体来源的效应细胞介导。ADCC-SRBC效应细胞的特征与ADCC-P815效应细胞不同。
