Ojo E, Wigzell H
Scand J Immunol. 1978 Apr;7(4):297-306. doi: 10.1111/j.1365-3083.1978.tb00457.x.
Mouse normal lymphoid cells were analysed as to their ability to perform in three cytolytic systems: Ability to act as 'natural killer', NK, cells against a NK sensitive tumour target, YAC; as effector cells against IgG-coated 815 cells, or to function as effector cells against IgG-coated CRBC. NK activity and ADCC against the IgG-coated P815 cells were found to vary in parallel as affected by age, organ distribution and genotype of the effector cells. On the other hand, ADCC against CRBC was largely carried out by effector cells distinct from those functioning as NK cells or in ADCC against P815. Temperature pretreatment schedules at 37 degrees C showed both NK cells and ADCC ability against P815 to be highly sensitive on contrast to ADCC against CRBC. Likewise, inoculation of Corynebacterium parvum intraperitoneally will lead to reduction in ADCC ability against CRBC but increase in ADCC against P815 and NK activity. Blocking experiments using 'cold' inhibitor cells in the cytolytic assays indicated that NK cells and effector cells against IgG-coated P815 cells are the very same cells. We thus conclude that NK cells in the mouse also have the ability to express K cell activity against IgG-coated tumour target cells. In fact, our data suggest that the NK cells may be the only cell type in the mouse equipped with cytolytic potential for antibody-coated murine nucleated cells
作为“自然杀伤”（NK）细胞对抗NK敏感肿瘤靶标YAC的能力；作为效应细胞对抗IgG包被的815细胞的能力，或作为效应细胞对抗IgG包被的CRBC的能力。发现针对IgG包被的P815细胞的NK活性和抗体依赖性细胞介导的细胞毒性（ADCC）会随着效应细胞的年龄、器官分布和基因型的影响而平行变化。另一方面，针对CRBC的ADCC主要由与作为NK细胞或针对P815进行ADCC的细胞不同的效应细胞来执行。37℃的温度预处理方案表明，与针对CRBC的ADCC相比，NK细胞和针对P815的ADCC能力对温度高度敏感。同样，腹腔内接种微小棒状杆菌会导致针对CRBC的ADCC能力降低，但针对P815的ADCC和NK活性增加。在细胞溶解试验中使用“冷”抑制细胞进行的阻断实验表明，NK细胞和针对IgG包被的P815细胞的效应细胞是同一类细胞。因此，我们得出结论，小鼠中的NK细胞也具有针对IgG包被的肿瘤靶细胞表达K细胞活性的能力。事实上，我们的数据表明，NK细胞可能是小鼠中唯一具有针对抗体包被的鼠有核细胞的细胞溶解潜能的细胞类型。
