Cervantes-Pérez Daniela, Ortega-García Angélica, Medina-Andrés Rigoberto, Batista-García Ramón Alberto, Lira-Ruan Verónica
Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos. Av. Universidad 1001. Col Chamilpa. Cuernavaca, Morelos 62210, Mexico.
Plants (Basel). 2020 Oct 16;9(10):1380. doi: 10.3390/plants9101380.
Nitric oxide (NO) has been recognized as a major player in the regulation of plant physiology and development. NO regulates cell cycle progression and cell elongation in flowering plants and green algae, although the information about NO function in non-vascular plants is scarce. Here, we analyze the effect of exogenous NO on protonema growth. We find that increasing concentrations of the NO donor sodium nitroprusside (SNP) inhibit protonema relative growth rate and cell length. To further comprehend the effect of NO on moss development, we analyze the effect of SNP 5 and 10 µM on protoplast regeneration and, furthermore, protonema formation compared with untreated plants (control). Isolated protoplasts were left to regenerate for 24 h before starting the SNP treatments that lasted five days. The results show that SNP restrains the protoplast regeneration process and the formation of new protonema cells. When SNP treatments started five days after protoplast isolation, a decrease in cell number per protonema filament was observed, indicating an inhibition of cell cycle progression. Our results show that in non-vascular plants, NO negatively regulates plant regeneration, cell cycle and cell elongation.
一氧化氮（NO）已被公认为植物生理和发育调控中的主要参与者。NO调节开花植物和绿藻中的细胞周期进程和细胞伸长，尽管关于NO在非维管植物中功能的信息较少。在此，我们分析了外源NO对原丝体生长的影响。我们发现，一氧化氮供体硝普钠（SNP）浓度的增加会抑制原丝体的相对生长速率和细胞长度。为了进一步理解NO对苔藓发育的影响，我们分析了5和10µM的SNP对原生质体再生的影响，此外，还分析了与未处理植物（对照）相比的原丝体形成情况。分离的原生质体在开始持续五天的SNP处理之前先进行24小时的再生。结果表明，SNP抑制原生质体再生过程和新原丝体细胞的形成。当在原生质体分离五天后开始SNP处理时，观察到每个原丝体细丝中的细胞数量减少，表明细胞周期进程受到抑制。我们的结果表明，在非维管植物中NO对植物再生、细胞周期和细胞伸长具有负调控作用。
