Yao Qi, Zhang Huining, Jiao Long, Cai Xiaoming, Wang Manqun, Chen Zongmao
Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Insects. 2020 Oct 16;11(10):707. doi: 10.3390/insects11100707.
Tea leafhopper ( Matsuda) is amongst the key pests in tea plantations around the East Asian region. Stereomicroscopy is a conventional method used for detecting tea leafhopper eggs by dissecting the tender tissues. However, there is a need for a faster and more efficient method to directly observe and investigate intact eggs within tea shoots. The absence of a proven method limits research efforts for determining the oviposition behavior of E. onukii. Herein, we applied the blue light detection method (BLDM), a technique recently developed for other species, in order to detect eggs directly and non-destructively within the tender shoot. In addition, we compared BLDM against the traditional stereomicroscope detection method (SMDM) for four tea cultivars. Notably, our results revealed that BLDM was precise and effective in measuring the egg laying quantity of on intact tea shoots. Neither tea cultivars nor egg density in the tender shoot significantly affected the accuracy of BLDM. Furthermore, biological characteristics that have rarely been reported previously for E. onukii were investigated using the BLDM, including zygote duration, ovipositional rhythm, egg distribution within the tender shoot, and in different leaf positions, numbers of eggs laid by a single female daily, and laid by the entire generation. Therefore, these findings provide insights into the basic and theoretical evidence for the strategy and mechanism associated with the oviposition behavior of .
假眼小绿叶蝉（Matsuda）是东亚地区茶园的主要害虫之一。实体显微镜检查是一种通过解剖嫩组织来检测假眼小绿叶蝉卵的传统方法。然而，需要一种更快、更有效的方法来直接观察和研究茶梢内完整的卵。缺乏经过验证的方法限制了对茶假眼小绿叶蝉产卵行为的研究。在此，我们应用了蓝光检测法（BLDM），这是一种最近为其他物种开发的技术，以便直接且无损地检测嫩梢内的卵。此外，我们针对四个茶树品种，将蓝光检测法与传统的实体显微镜检测法（SMDM）进行了比较。值得注意的是，我们的结果表明，蓝光检测法在测量完整茶梢上的产卵量方面准确有效。茶树品种和嫩梢中的卵密度均未显著影响蓝光检测法的准确性。此外，利用蓝光检测法研究了茶假眼小绿叶蝉以前很少报道的生物学特性，包括合子持续时间、产卵节律、嫩梢内以及不同叶位的卵分布、单雌每日产卵数以及全代产卵数。因此，这些发现为茶假眼小绿叶蝉产卵行为相关的策略和机制提供了基础和理论依据。
